Detailed information of TA system TA032887

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 861307..861964
• Replicon: chromosome
• Accession: NZ_AP019687
• Organism: Klebsiella quasipneumoniae strain SNI47

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A5D1WGJ3
• Locus tag: TMSI_RS04335
• Protein ID: WP_072420599.1
• Coordinates: 861554..861964 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: TMSI_RS04330
• Protein ID: WP_101862175.1
• Coordinates: 861307..861573 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TMSI_RS04305	856457..857890	-	1434	WP_023290972.1	6-phospho-beta-glucosidase BglA	-
TMSI_RS04310	858009..858737	-	729	WP_032454033.1	MurR/RpiR family transcriptional regulator	-
TMSI_RS04315	858788..859099	+	312	WP_023290970.1	N(4)-acetylcytidine aminohydrolase	-
TMSI_RS04320	859263..859922	+	660	WP_008806429.1	hemolysin III family protein	-
TMSI_RS04325	860078..861061	-	984	WP_101862176.1	tRNA-modifying protein YgfZ	-
TMSI_RS04330	861307..861573	+	267	WP_101862175.1	FAD assembly factor SdhE	Antitoxin
TMSI_RS04335	861554..861964	+	411	WP_072420599.1	protein YgfX	Toxin
TMSI_RS04340	861971..862492	-	522	WP_004205324.1	flavodoxin FldB	-
TMSI_RS04345	862593..863489	+	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
TMSI_RS04350	863512..864225	+	714	WP_101862174.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
TMSI_RS04355	864231..865964	+	1734	WP_032454029.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16089.92 Da
• Isoelectric Point: 11.4778

>T32887 WP_072420599.1 NZ_AP019687:861554-861964 [Klebsiella quasipneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWIINSGMLLRLRHVDTRRGQHLWLAADSMDPGEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T32887 NZ_AP019687:861554-861964 [Klebsiella quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAATGCC
GAATGGGAGATCCTCGGGACGCCGTGGATCATCAACAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGACGCGG
TCAGCATCTGTGGCTGGCGGCGGACAGCATGGATCCCGGAGAGTGGCGGGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10511.01 Da
• Isoelectric Point: 5.1116

>AT32887 WP_101862175.1 NZ_AP019687:861307-861573 [Klebsiella quasipneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADTELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT32887 NZ_AP019687:861307-861573 [Klebsiella quasipneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTTAACTGGCTGATGAACCACGGCAAACCGGCCGATACGGAGCTGCAGCGGATGGTAACCTTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A5D1WGJ3