Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3454107..3454725 Replicon chromosome
Accession NZ_AP019675
Organism Escherichia coli strain GSH8M-2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GDK32_RS17010 Protein ID WP_001291435.1
Coordinates 3454507..3454725 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GDK32_RS17005 Protein ID WP_000344800.1
Coordinates 3454107..3454481 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GDK32_RS16995 3449196..3450389 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GDK32_RS17000 3450412..3453561 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
GDK32_RS17005 3454107..3454481 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GDK32_RS17010 3454507..3454725 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GDK32_RS17015 3454897..3455448 + 552 WP_000102564.1 maltose O-acetyltransferase -
GDK32_RS17020 3455564..3456034 + 471 WP_000136192.1 YlaC family protein -
GDK32_RS17025 3456198..3457748 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GDK32_RS17030 3457790..3458143 - 354 WP_000878140.1 DUF1428 domain-containing protein -
GDK32_RS17040 3458522..3458833 + 312 WP_182916815.1 MGMT family protein -
GDK32_RS17045 3458864..3459436 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T32878 WP_001291435.1 NZ_AP019675:3454507-3454725 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T32878 NZ_AP019675:3454507-3454725 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT32878 WP_000344800.1 NZ_AP019675:3454107-3454481 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT32878 NZ_AP019675:3454107-3454481 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References