Detailed information of TA system TA032877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2464520..2465158
• Replicon: chromosome
• Accession: NZ_AP019675
• Organism: Escherichia coli strain GSH8M-2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: GDK32_RS12095
• Protein ID: WP_000813794.1
• Coordinates: 2464982..2465158 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GDK32_RS12090
• Protein ID: WP_001270286.1
• Coordinates: 2464520..2464936 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GDK32_RS12070	2459672..2460613	-	942	WP_001251313.1	ABC transporter permease	-
GDK32_RS12075	2460614..2461627	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
GDK32_RS12080	2461645..2462790	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
GDK32_RS12085	2463035..2464441	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
GDK32_RS12090	2464520..2464936	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
GDK32_RS12095	2464982..2465158	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
GDK32_RS12100	2465380..2465610	+	231	WP_000494244.1	YncJ family protein	-
GDK32_RS12105	2465702..2467663	-	1962	WP_001752477.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
GDK32_RS12110	2467736..2468272	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
GDK32_RS12115	2468325..2469554	+	1230	WP_071531711.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2469594..2470742	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T32877 WP_000813794.1 NZ_AP019675:c2465158-2464982 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T32877 NZ_AP019675:c2465158-2464982 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT32877 WP_001270286.1 NZ_AP019675:c2464936-2464520 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT32877 NZ_AP019675:c2464936-2464520 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB