Detailed information of TA system TA032854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2712810..2713561
• Replicon: chromosome
• Accession: NZ_AP019669
• Organism: Raoultella ornithinolytica strain GSH0205-8M-1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: QMG92_RS12765
• Protein ID: WP_044351088.1
• Coordinates: 2713079..2713561 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A808Y4Y8
• Locus tag: QMG92_RS12760
• Protein ID: WP_015584326.1
• Coordinates: 2712810..2713088 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG92_RS12750 (ROGSH02058M1_024980)	2709537..2710085	+	549	WP_227819043.1	DUF4451 domain-containing protein	-
QMG92_RS12755 (ROGSH02058M1_024990)	2710243..2712168	-	1926	WP_064359428.1	C1 family peptidase	-
QMG92_RS12760 (ROGSH02058M1_025000)	2712810..2713088	+	279	WP_015584326.1	DUF1778 domain-containing protein	Antitoxin
QMG92_RS12765 (ROGSH02058M1_025010)	2713079..2713561	+	483	WP_044351088.1	GNAT family N-acetyltransferase	Toxin
QMG92_RS12770	2713680..2714046	-	367	Protein_2497	ISNCY family transposase	-
QMG92_RS12775 (ROGSH02058M1_025030)	2714228..2714860	+	633	WP_001567369.1	hypothetical protein	-
QMG92_RS12780 (ROGSH02058M1_025040)	2714889..2716292	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
QMG92_RS12785 (ROGSH02058M1_025050)	2716486..2716644	-	159	WP_155728879.1	hypothetical protein	-
QMG92_RS12790 (ROGSH02058M1_025060)	2717363..2718238	-	876	WP_032717451.1	NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2683236..2859883	176647
	-	flank	IS/Tn	-	-	2714889..2716292	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17612.48 Da
• Isoelectric Point: 9.7381

>T32854 WP_044351088.1 NZ_AP019669:2713079-2713561 [Raoultella ornithinolytica]
MGMRPPEPLSAGHNIAEFCCQDQVLNEWLKKKALKNHSTGISRVFVVCAENTKRVIAYYSLSSGSVHRNTVPGAYRRNAP
EAVPVIVLGRLAVDAAWAGKGLGAALLKDAIYRTEHIAVQVGVRALLVHALNDEVREFYTRFGFEPSIINTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T32854 NZ_AP019669:2713079-2713561 [Raoultella ornithinolytica]
ATGGGAATGAGACCACCGGAACCACTCAGCGCTGGACACAACATAGCAGAATTTTGTTGTCAGGATCAGGTTCTTAACGA
GTGGTTGAAGAAGAAGGCGCTGAAGAACCACAGTACAGGCATTTCAAGGGTCTTTGTGGTCTGTGCCGAAAACACAAAAA
GGGTCATCGCGTATTACAGCTTATCATCCGGGAGCGTGCACAGAAACACAGTACCGGGGGCATACCGTCGTAATGCACCT
GAAGCTGTACCGGTCATTGTTCTGGGAAGGCTTGCGGTCGATGCAGCCTGGGCAGGGAAGGGACTCGGAGCGGCACTGCT
TAAGGATGCAATATACCGTACAGAACATATCGCAGTTCAGGTCGGTGTAAGGGCGCTTCTCGTCCATGCCTTGAATGATG
AAGTCAGGGAGTTTTACACCAGGTTTGGATTCGAGCCCTCGATTATCAATACACTGACATTATTGTTCCCGATAAAAGTA
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10714.30 Da
• Isoelectric Point: 4.6722

>AT32854 WP_015584326.1 NZ_AP019669:2712810-2713088 [Raoultella ornithinolytica]
MKPDVREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDKQFASFMEELDAPMTAERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT32854 NZ_AP019669:2712810-2713088 [Raoultella ornithinolytica]
ATGAAACCAGACGTTCGTGAAGCTCCGATAAACATTCGGGCGAAAACATCCCAGCGCGACCTTATCGACATGGCCGCAAA
TCTTGTATCTAAATCACGTACTGATTTTATGCTTGAAGTGGCCTGTCGCGAAGCTCAGGACATTCTGTTGGATCAGCGCT
TGTTCCTGCTGGACGATAAGCAGTTTGCTAGTTTCATGGAAGAGCTTGACGCACCTATGACAGCGGAGCGTCAGGCACGT
ATTGATAACCTGATGAACCGCAAATCGCCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A808Y4Y8