Detailed information of TA system TA032843

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 923165..923970
• Replicon: chromosome
• Accession: NZ_AP019669
• Organism: Raoultella ornithinolytica strain GSH0205-8M-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7L6ICB7
• Locus tag: QMG92_RS04505
• Protein ID: WP_049238634.1
• Coordinates: 923581..923970 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: QMG92_RS04500
• Protein ID: WP_049238636.1
• Coordinates: 923165..923524 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG92_RS04470 (ROGSH02058M1_008810)	919640..920272	-	633	WP_154955094.1	inovirus Gp2 family protein	-
QMG92_RS04475 (ROGSH02058M1_008820)	920813..921637	+	825	WP_183114509.1	DUF932 domain-containing protein	-
QMG92_RS04480 (ROGSH02058M1_008830)	921634..921891	+	258	WP_049238645.1	DUF905 domain-containing protein	-
QMG92_RS04485 (ROGSH02058M1_008840)	921971..922414	+	444	WP_049238643.1	antirestriction protein	-
QMG92_RS04490 (ROGSH02058M1_008850)	922426..922905	+	480	WP_049238640.1	DNA repair protein RadC	-
QMG92_RS04495 (ROGSH02058M1_008860)	922919..923140	+	222	WP_049238638.1	DUF987 domain-containing protein	-
QMG92_RS04500 (ROGSH02058M1_008870)	923165..923524	+	360	WP_049238636.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
QMG92_RS04505 (ROGSH02058M1_008880)	923581..923970	+	390	WP_049238634.1	TA system toxin CbtA family protein	Toxin
QMG92_RS04510 (ROGSH02058M1_008890)	924057..924896	+	840	WP_049238632.1	DUF4942 domain-containing protein	-
QMG92_RS04515 (ROGSH02058M1_008900)	925224..926831	+	1608	WP_100169050.1	site-specific integrase	-
QMG92_RS04520 (ROGSH02058M1_008910)	926832..927464	+	633	WP_077070083.1	DUF4145 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (3-111)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14387.45 Da
• Isoelectric Point: 9.6254

>T32843 WP_049238634.1 NZ_AP019669:923581-923970 [Raoultella ornithinolytica]
MQTKLSPLMRAASSRPSPVGVWQTLLTCLLEHHYGLTLNDTPFSDEQVIQQHIDAGISLSDALNFIVEKYELVRTDRPGF
SIREQSPFITPIDILRARKAAGLMNRGTYKEVTAITRGQHPQANASGKR

Nucleotide

• Length: 390 bp

>T32843 NZ_AP019669:923581-923970 [Raoultella ornithinolytica]
ATGCAAACCAAATTATCCCCCCTCATGCGGGCGGCATCGTCACGCCCGTCTCCCGTGGGTGTCTGGCAGACCCTGCTGAC
CTGCCTGCTGGAACATCACTATGGTCTCACGCTTAACGATACGCCGTTCAGTGATGAACAGGTGATTCAGCAGCATATCG
ACGCAGGTATCTCGCTGTCCGATGCCCTGAATTTTATTGTAGAAAAGTATGAACTGGTCCGCACTGACCGGCCAGGCTTC
AGCATCAGGGAGCAGTCTCCGTTTATCACACCCATTGATATCCTCCGGGCCAGAAAGGCTGCGGGGCTGATGAACCGTGG
CACGTATAAAGAGGTGACGGCTATTACCAGAGGACAACATCCGCAGGCCAACGCTTCAGGCAAGCGATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13419.27 Da
• Isoelectric Point: 6.9557

>AT32843 WP_049238636.1 NZ_AP019669:923165-923524 [Raoultella ornithinolytica]
MSKKTTITTHDISEPWWGLRRSVSPCFGARLVQERNSLHYLADRANFDGQFCDADLRYLDQAFPVLMKQLELMLTSGELN
PRHQHGVTLYAKGLTCEADTLGSHGYVYIAIYPTPVTTA

Nucleotide

• Length: 360 bp

>AT32843 NZ_AP019669:923165-923524 [Raoultella ornithinolytica]
TTGTCGAAGAAAACCACGATAACAACGCATGATATCAGCGAACCCTGGTGGGGGCTCAGACGCAGTGTCTCCCCGTGTTT
TGGGGCCCGTCTGGTCCAGGAGAGAAACTCCCTGCATTACCTGGCTGACCGTGCGAACTTTGATGGTCAGTTCTGCGATG
CGGATTTGCGCTATCTTGACCAGGCCTTCCCGGTACTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAAT
CCCCGTCATCAGCACGGCGTCACACTGTATGCAAAAGGTCTGACCTGCGAGGCGGATACGCTCGGTTCGCACGGCTACGT
TTATATCGCGATTTATCCCACTCCCGTCACCACGGCATAA

Structures

Toxin

• AlphaFold DB: A0A7L6ICB7