Detailed information of TA system TA032840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 84801..85711
• Replicon: plasmid pTMTA63631
• Accession: NZ_AP019666
• Organism: Klebsiella pneumoniae strain TA6363

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: TA6363_RS26430
• Protein ID: WP_004026354.1
• Coordinates: 84801..85271 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: TA6363_RS26435
• Protein ID: WP_004026357.1
• Coordinates: 85268..85711 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TA6363_RS26410	79876..80274	-	399	WP_004212796.1	H-NS histone family protein	-
TA6363_RS26415	80611..81105	-	495	WP_004212794.1	thermonuclease family protein	-
TA6363_RS26420	81283..82232	+	950	Protein_79	DUF1173 family protein	-
TA6363_RS26425	83618..84315	-	698	Protein_80	IS1 family transposase	-
TA6363_RS26430	84801..85271	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
TA6363_RS26435	85268..85711	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
TA6363_RS26440	85812..86315	-	504	Protein_83	DUF4113 domain-containing protein	-
TA6363_RS26445	86413..86634	-	222	WP_004213643.1	DUF2188 domain-containing protein	-
TA6363_RS26450	86779..86934	-	156	WP_004213642.1	hypothetical protein	-
TA6363_RS26455	87009..89135	-	2127	WP_004213640.1	TonB-dependent receptor family protein	-
TA6363_RS26460	89222..90175	-	954	Protein_87	fec operon regulator FecR	-
TA6363_RS26465	90172..90693	-	522	WP_004213635.1	sigma-70 family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / pla	1..166700	166700

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T32840 WP_004026354.1 NZ_AP019666:c85271-84801 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T32840 NZ_AP019666:c85271-84801 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT32840 WP_004026357.1 NZ_AP019666:c85711-85268 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT32840 NZ_AP019666:c85711-85268 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9