Detailed information of TA system TA032838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5240605..5241230
• Replicon: chromosome
• Accession: NZ_AP019665
• Organism: Klebsiella pneumoniae strain TA6363

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: TA6363_RS25640
• Protein ID: WP_163584268.1
• Coordinates: 5240605..5240988 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: TA6363_RS25645
• Protein ID: WP_004150355.1
• Coordinates: 5240988..5241230 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TA6363_RS25625	5237971..5238873	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
TA6363_RS25630	5238870..5239505	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
TA6363_RS25635	5239502..5240431	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
TA6363_RS25640	5240605..5240988	-	384	WP_163584268.1	type II toxin-antitoxin system VapC family toxin	Toxin
TA6363_RS25645	5240988..5241230	-	243	WP_004150355.1	ribbon-helix-helix domain-containing protein	Antitoxin
TA6363_RS25650	5241435..5242352	+	918	WP_004178029.1	alpha/beta hydrolase	-
TA6363_RS25655	5242366..5243307	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
TA6363_RS25660	5243352..5243789	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
TA6363_RS25665	5243786..5244646	-	861	WP_004146232.1	virulence factor BrkB family protein	-
TA6363_RS25670	5244640..5245239	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14349.56 Da
• Isoelectric Point: 6.7269

>T32838 WP_163584268.1 NZ_AP019665:c5240988-5240605 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTQMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T32838 NZ_AP019665:c5240988-5240605 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCAAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT32838 WP_004150355.1 NZ_AP019665:c5241230-5240988 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT32838 NZ_AP019665:c5241230-5240988 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9