Detailed information of TA system TA032821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-YefM
• Location: 10751..11310
• Replicon: plasmid pVPon1
• Accession: NZ_AP019659
• Organism: Vibrio ponticus strain DSM 16217

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: GZN30_RS21100
• Protein ID: WP_075647684.1
• Coordinates: 11011..11310 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: GZN30_RS21095
• Protein ID: WP_075647683.1
• Coordinates: 10751..11011 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZN30_RS21075	6455..7645	-	1191	WP_167521327.1	pyridoxal phosphate-dependent aminotransferase	-
GZN30_RS21080	7645..9093	-	1449	WP_075647680.1	hypothetical protein	-
GZN30_RS21085	9250..9711	-	462	WP_075647681.1	MarR family transcriptional regulator	-
GZN30_RS21090	9996..10544	-	549	WP_075647682.1	recombinase family protein	-
GZN30_RS21095	10751..11011	+	261	WP_075647683.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GZN30_RS21100	11011..11310	+	300	WP_075647684.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GZN30_RS21105	11288..11911	-	624	WP_075647685.1	recombinase family protein	-
GZN30_RS21110	12502..13578	-	1077	WP_075652691.1	replication initiation protein	-
GZN30_RS21115	14528..15250	+	723	WP_075652701.1	hypothetical protein	-
GZN30_RS21120	15258..16001	+	744	WP_075652702.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48011	48011

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-57)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11493.27 Da
• Isoelectric Point: 8.8454

>T32821 WP_075647684.1 NZ_AP019659:11011-11310 [Vibrio ponticus]
MAQYKLSPAAQTDLIDIRRYTLENWGSVQWTNYFNELKQSMTLLANNELIGIDMPELGAGYCRFPLKHHVIYYIRKPDHI
VIAAVLGRNMSPAKHFQQQ

Nucleotide

• Length: 300 bp

>T32821 NZ_AP019659:11011-11310 [Vibrio ponticus]
ATGGCACAATACAAACTTTCTCCTGCCGCACAGACCGATCTTATCGATATCCGCCGCTACACACTCGAAAACTGGGGAAG
TGTGCAATGGACTAACTATTTCAATGAACTCAAACAATCAATGACGTTGTTAGCCAATAACGAATTAATTGGTATCGACA
TGCCCGAACTGGGTGCGGGTTATTGCCGCTTTCCTTTAAAGCATCATGTGATTTACTACATTAGAAAACCGGACCATATT
GTTATCGCCGCGGTATTGGGTCGCAATATGTCCCCTGCTAAGCACTTTCAGCAACAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9636.02 Da
• Isoelectric Point: 4.3322

>AT32821 WP_075647683.1 NZ_AP019659:10751-11011 [Vibrio ponticus]
MQSLTANTAKTKFGDLLMKVQREPVQISKNGSPVAVMMSCEEYAELEALKMAMVKSRFEQAERDIANDNLVDGDTFMNEL
DLGKFD

Nucleotide

• Length: 261 bp

>AT32821 NZ_AP019659:10751-11011 [Vibrio ponticus]
ATGCAATCATTAACCGCTAATACGGCAAAAACTAAATTTGGTGACCTACTTATGAAGGTACAGCGTGAGCCTGTACAAAT
CAGCAAAAATGGTTCACCGGTGGCAGTCATGATGTCATGCGAAGAATATGCAGAGCTTGAAGCTTTAAAGATGGCCATGG
TGAAGTCGCGTTTTGAGCAAGCGGAGCGAGATATCGCGAATGATAATTTGGTCGATGGCGATACCTTCATGAACGAATTG
GATCTAGGCAAGTTTGACTAA