Detailed information of TA system TA032801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 4561851..4562467
• Replicon: chromosome
• Accession: NZ_AP019632
• Organism: Enterobacter asburiae strain 1808-013

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FOY96_RS21850
• Protein ID: WP_096151167.1
• Coordinates: 4561851..4562222 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A808IJD0
• Locus tag: FOY96_RS21855
• Protein ID: WP_023309753.1
• Coordinates: 4562225..4562467 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOY96_RS21835	4559351..4560253	+	903	WP_008501831.1	formate dehydrogenase subunit beta	-
FOY96_RS21840	4560250..4560885	+	636	WP_010436852.1	formate dehydrogenase cytochrome b556 subunit	-
FOY96_RS21845	4560882..4561811	+	930	WP_029741588.1	formate dehydrogenase accessory protein FdhE	-
FOY96_RS21850	4561851..4562222	-	372	WP_096151167.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOY96_RS21855	4562225..4562467	-	243	WP_023309753.1	ribbon-helix-helix protein, CopG family	Antitoxin
FOY96_RS21860	4562667..4563587	+	921	WP_096151166.1	alpha/beta hydrolase	-
FOY96_RS21865	4563596..4564537	-	942	WP_039264191.1	fatty acid biosynthesis protein FabY	-
FOY96_RS21870	4564582..4565019	-	438	WP_033146964.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FOY96_RS21875	4565016..4565897	-	882	WP_033146965.1	virulence factor BrkB family protein	-
FOY96_RS21880	4565891..4566490	-	600	WP_143347721.1	glucose-1-phosphatase	-
FOY96_RS21885	4566579..4567049	-	471	Protein_4225	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13702.87 Da
• Isoelectric Point: 6.9789

>T32801 WP_096151167.1 NZ_AP019632:c4562222-4561851 [Enterobacter asburiae]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMKLPDAIILATAQSRNCPLISRNTKDFTGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T32801 NZ_AP019632:c4562222-4561851 [Enterobacter asburiae]
ATGGAGCACATGGCGGTGTTCGATACCAATATACTCATCGATCTTTTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCGCATCGAGCCATTAGTCTGATCACATGGATGGAAGTTATGGTGGGGGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTCTGCGC
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAATTGTCCGTTGATTTCTCGAAA
CACGAAAGACTTTACGGGCATTACTGGAGTCGTTTCACCTTACCAACTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9429.56 Da
• Isoelectric Point: 4.2803

>AT32801 WP_023309753.1 NZ_AP019632:c4562467-4562225 [Enterobacter asburiae]
MSLSADLDMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT32801 NZ_AP019632:c4562467-4562225 [Enterobacter asburiae]
ATGAGTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGATGACGTAATTCAACGACTGGATAATCT
GAAGCAGCTGCGTAACCGGCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTTGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCTTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A808IJD0