Detailed information of TA system TA032785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 253240..253836
• Replicon: chromosome
• Accession: NZ_AP019632
• Organism: Enterobacter asburiae strain 1808-013

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A4R0G5K0
• Locus tag: FOY96_RS01135
• Protein ID: WP_039264167.1
• Coordinates: 253534..253836 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: FOY96_RS01130
• Protein ID: WP_096151293.1
• Coordinates: 253240..253527 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOY96_RS01125	251612..253243	+	1632	WP_033144374.1	Na/Pi cotransporter family protein	-
FOY96_RS01130	253240..253527	-	288	WP_096151293.1	putative addiction module antidote protein	Antitoxin
FOY96_RS01135	253534..253836	-	303	WP_039264167.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOY96_RS01140	254034..254906	+	873	WP_039264166.1	23S rRNA pseudouridine(2604) synthase RluF	-
FOY96_RS01145	254907..255179	-	273	WP_023309994.1	DUF3811 domain-containing protein	-
FOY96_RS01150	255230..256174	-	945	WP_143346388.1	ketopantoate/pantoate/pantothenate transporter PanS	-
FOY96_RS01155	256268..257617	-	1350	WP_023309996.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11401.17 Da
• Isoelectric Point: 9.6038

>T32785 WP_039264167.1 NZ_AP019632:c253836-253534 [Enterobacter asburiae]
MKEIVQTESFQRWEQNLKDRRAKTIIASRLFRLANGLAGDIKPVGEGISELRIHYGPGYRIYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T32785 NZ_AP019632:c253836-253534 [Enterobacter asburiae]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCAACGCTGGGAGCAAAACTTAAAAGATCGGCGAGCGAAAACCATTATCGC
TTCCCGCCTCTTTCGGTTGGCAAATGGTTTAGCGGGCGACATTAAACCCGTGGGCGAAGGTATAAGTGAGCTGAGGATCC
ACTATGGTCCGGGCTACAGAATCTATTTTAAAGACCAGGGCAATTGCATCATCGTGCTGTTGTGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10183.66 Da
• Isoelectric Point: 5.6557

>AT32785 WP_096151293.1 NZ_AP019632:c253527-253240 [Enterobacter asburiae]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKHAED

Nucleotide

• Length: 288 bp

>AT32785 NZ_AP019632:c253527-253240 [Enterobacter asburiae]
ATGCATAAATTAACACCCTACGATCCAGCCAATGCGCTGGTGGATGACGAGGAAATCGCCGTGTTTATGGCTGATGCCTT
AGAAACGGGCGACTCAGCGTATATTGCTAAAGCTCTGGGCGTCATCGCGCGAGCGAAAGGAATGTCGACCATTTCACAGC
AAACTGGCCTATCACGAGAGCAACTGTATCGATCGTTCAGTGATAAAGGAAACCCAACGCTTAAAACCACTCTGGCGGTC
ATGAAAGCATTGGGTCTTGGGCTAACAATCAAACACGCCGAAGATTAA

Structures

Toxin

• AlphaFold DB: A0A4R0G5K0