Detailed information of TA system TA032784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 4565298..4565914
• Replicon: chromosome
• Accession: NZ_AP019630
• Organism: Enterobacter asburiae strain 17Nkhm-UP2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FOY94_RS21975
• Protein ID: WP_080298642.1
• Coordinates: 4565298..4565669 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A808IJD0
• Locus tag: FOY94_RS21980
• Protein ID: WP_023309753.1
• Coordinates: 4565672..4565914 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOY94_RS21960	4562798..4563700	+	903	WP_008501831.1	formate dehydrogenase subunit beta	-
FOY94_RS21965	4563697..4564332	+	636	WP_010436852.1	formate dehydrogenase cytochrome b556 subunit	-
FOY94_RS21970	4564329..4565258	+	930	WP_029741588.1	formate dehydrogenase accessory protein FdhE	-
FOY94_RS21975	4565298..4565669	-	372	WP_080298642.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOY94_RS21980	4565672..4565914	-	243	WP_023309753.1	ribbon-helix-helix protein, CopG family	Antitoxin
FOY94_RS21985	4566114..4567034	+	921	WP_048981029.1	alpha/beta hydrolase	-
FOY94_RS21990	4567043..4567984	-	942	WP_039264191.1	fatty acid biosynthesis protein FabY	-
FOY94_RS21995	4568029..4568466	-	438	WP_008501836.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FOY94_RS22000	4568463..4569344	-	882	WP_033146965.1	virulence factor BrkB family protein	-
FOY94_RS22005	4569338..4569937	-	600	WP_039264189.1	glucose-1-phosphatase	-
FOY94_RS22010	4570026..4570496	-	471	WP_033146967.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13688.85 Da
• Isoelectric Point: 6.9789

>T32784 WP_080298642.1 NZ_AP019630:c4565669-4565298 [Enterobacter asburiae]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMKLPDAIILATAQSRNCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T32784 NZ_AP019630:c4565669-4565298 [Enterobacter asburiae]
ATGGAGCACATGGCAGTGTTCGATACCAATATACTCATCGATCTTTTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCTCATCGAGCCATTAGTCTGATCACATGGATGGAAGTCATGGTGGGGGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTCTGCGC
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAATTGTCCGTTGATTTCTCGAAA
CACGAAAGACTTTTCGGGCATTACTGGAGTCGTTTCACCTTACCAACTATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9411.53 Da
• Isoelectric Point: 4.2803

>AT32784 WP_023309753.1 NZ_AP019630:c4565914-4565672 [Enterobacter asburiae]
ISLSADLDMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT32784 NZ_AP019630:c4565914-4565672 [Enterobacter asburiae]
ATAAGTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGATGACGTAATTCAACGACTGGATAATCT
GAAGCAGCTGCGTAACCGTCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTCGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCTTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A808IJD0