Detailed information of TA system TA032781

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 3571282..3572015
• Replicon: chromosome
• Accession: NZ_AP019630
• Organism: Enterobacter asburiae strain 17Nkhm-UP2

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: FOY94_RS17110
• Protein ID: WP_143346148.1
• Coordinates: 3571686..3572015 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FOY94_RS17105
• Protein ID: WP_143346147.1
• Coordinates: 3571282..3571647 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOY94_RS17070	3567191..3567625	+	435	WP_109948925.1	hypothetical protein	-
FOY94_RS17075	3567685..3568524	+	840	WP_143346144.1	hypothetical protein	-
FOY94_RS17080	3568583..3568774	+	192	WP_041412185.1	hypothetical protein	-
FOY94_RS17085	3569188..3570009	+	822	WP_143346145.1	DUF945 domain-containing protein	-
FOY94_RS17090	3570040..3570483	+	444	WP_134871855.1	antirestriction protein	-
FOY94_RS17095	3570496..3571038	+	543	WP_143346146.1	DNA repair protein RadC	-
FOY94_RS17100	3571035..3571256	+	222	WP_109948919.1	DUF987 domain-containing protein	-
FOY94_RS17105	3571282..3571647	+	366	WP_143346147.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FOY94_RS17110	3571686..3572015	+	330	WP_143346148.1	TA system toxin CbtA family protein	Toxin
FOY94_RS17115	3572334..3572552	-	219	WP_045334934.1	ogr/Delta-like zinc finger family protein	-
FOY94_RS17120	3572621..3573172	-	552	Protein_3308	phage late control D family protein	-
FOY94_RS17125	3573212..3574384	-	1173	WP_007848866.1	phage tail sheath protein	-
FOY94_RS17130	3574582..3575628	+	1047	WP_143346149.1	acyltransferase	-
FOY94_RS17135	3575620..3576222	-	603	WP_143346150.1	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3559439..3604252	44813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12418.17 Da
• Isoelectric Point: 7.2752

>T32781 WP_143346148.1 NZ_AP019630:3571686-3572015 [Enterobacter asburiae]
MQTISSHPTRATQPCLSPVEIWQRLITYLLLQHYGLTLNDTPFSNDSTIQEHIDAGISLSDAVNFLVEKYGLVRIDRKGF
SWQEQTPYLSVVDILRARRSTGLLKTDVK

Nucleotide

• Length: 330 bp

>T32781 NZ_AP019630:3571686-3572015 [Enterobacter asburiae]
ATGCAAACCATATCATCACACCCGACACGGGCGACTCAGCCCTGCCTGTCACCTGTTGAAATCTGGCAGCGGCTGATAAC
TTATCTGTTGTTGCAGCACTATGGCCTGACACTGAATGACACACCGTTCAGCAATGACTCCACCATCCAGGAACATATTG
ATGCTGGAATATCGCTCAGTGATGCAGTGAACTTTCTGGTAGAAAAATACGGGCTTGTCCGTATCGATCGGAAAGGATTT
TCGTGGCAAGAACAAACTCCGTATCTTTCCGTAGTGGATATTTTGCGAGCAAGACGCTCTACCGGCTTGTTAAAAACTGA
CGTGAAATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13615.40 Da
• Isoelectric Point: 5.5169

>AT32781 WP_143346147.1 NZ_AP019630:3571282-3571647 [Enterobacter asburiae]
MNNHSESGTKPESPARQLWGLKRDITPCFGARLVQEGNRLHYLADRAEFSGAFTDDVAMRLDQAFPLMMKQLELMLTSGE
LNPRHQHCVTLYHNGLACEADTLGSCGYVYIAIYPEQTEPQ

Nucleotide

• Length: 366 bp

>AT32781 NZ_AP019630:3571282-3571647 [Enterobacter asburiae]
ATGAACAATCACTCAGAATCCGGTACTAAGCCGGAGAGTCCCGCCCGCCAGTTGTGGGGACTGAAGCGCGATATAACACC
TTGTTTTGGGGCTCGTCTGGTTCAGGAAGGCAACCGACTGCACTATCTGGCTGACCGAGCAGAATTTAGCGGGGCGTTCA
CCGATGATGTTGCAATGCGCCTTGACCAGGCATTTCCGCTCATGATGAAACAACTTGAGCTGATGCTGACCAGTGGAGAA
CTTAACCCTCGCCACCAACACTGCGTTACGCTATACCACAACGGCCTCGCCTGCGAAGCCGACACACTCGGCTCCTGTGG
CTATGTCTACATTGCTATCTATCCTGAGCAGACTGAGCCGCAATAA