Detailed information of TA system TA032771

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): KacT-ataR/DUF1778(antitoxin)
• Location: 1671248..1672045
• Replicon: chromosome
• Accession: NZ_AP019630
• Organism: Enterobacter asburiae strain 17Nkhm-UP2

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: FOY94_RS07960
• Protein ID: WP_172620298.1
• Coordinates: 1671545..1672045 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: FOY94_RS07955
• Protein ID: WP_023311079.1
• Coordinates: 1671248..1671517 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOY94_RS07925	1666875..1667318	-	444	WP_032658095.1	helix-turn-helix transcriptional regulator	-
FOY94_RS07930	1667471..1668712	+	1242	WP_048979071.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
FOY94_RS07935	1668746..1668973	-	228	WP_008499832.1	YccJ family protein	-
FOY94_RS07940	1668994..1669590	-	597	WP_023311076.1	NAD(P)H:quinone oxidoreductase	-
FOY94_RS07945	1669981..1670151	+	171	WP_023311077.1	general stress protein	-
FOY94_RS07950	1670269..1671186	+	918	WP_048979072.1	DMT family transporter	-
FOY94_RS07955	1671248..1671517	+	270	WP_023311079.1	DUF1778 domain-containing protein	Antitoxin
FOY94_RS07960	1671545..1672045	+	501	WP_172620298.1	GNAT family N-acetyltransferase	Toxin
FOY94_RS07965	1672119..1673441	-	1323	WP_048979074.1	pyrimidine utilization transport protein G	-
FOY94_RS07970	1673463..1673957	-	495	WP_048979075.1	pyrimidine utilization flavin reductase protein F	-
FOY94_RS07975	1673967..1674557	-	591	WP_033145164.1	malonic semialdehyde reductase	-
FOY94_RS07980	1674567..1675367	-	801	WP_048979076.1	pyrimidine utilization protein D	-
FOY94_RS07985	1675375..1675761	-	387	WP_010429508.1	pyrimidine utilization protein C	-
FOY94_RS07990	1675773..1676462	-	690	WP_033145167.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (2-88)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 19018.74 Da
• Isoelectric Point: 7.9108

>T32771 WP_172620298.1 NZ_AP019630:1671545-1672045 [Enterobacter asburiae]
MLSEGTDYDFEYFDCGEPSLNAFLAEHLVRQHNGRILRAYLLKERDRPRVLGYYTLSGSCFERAMLPSKTQQRRIPYINV
PSVTLGRLAVDKTLQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALDERAKRFYLKQGFIPLTAENSHSLFFPTKSIE
RLFEQE

Nucleotide

• Length: 501 bp

>T32771 NZ_AP019630:1671545-1672045 [Enterobacter asburiae]
ATGTTATCAGAGGGAACCGATTATGACTTCGAGTATTTTGATTGTGGCGAACCCTCGCTAAATGCCTTCCTTGCTGAACA
TCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGCCTACCTGCTTAAAGAGCGCGATCGTCCGCGTGTCCTGGGTTATT
ACACGTTATCGGGAAGCTGTTTTGAAAGAGCGATGCTTCCTTCTAAGACTCAGCAGCGCCGGATCCCTTATATCAACGTA
CCCAGCGTGACGCTGGGACGTCTGGCTGTAGATAAAACCCTACAGGGAAACGAGTGGGGAACAACGCTCGTTGCGCATGC
TATGCGCGTTGTCTACCTGGCTTCACAGGCTGTGGGGGTACACGGAATATTTGTTGATGCCCTTGACGAGCGGGCAAAAC
GATTTTATCTGAAGCAGGGCTTTATCCCGCTGACAGCTGAAAACAGCCATTCTTTGTTCTTCCCTACAAAATCTATCGAA
CGGCTGTTTGAGCAAGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10249.72 Da
• Isoelectric Point: 5.8836

>AT32771 WP_023311079.1 NZ_AP019630:1671248-1671517 [Enterobacter asburiae]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWTAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT32771 NZ_AP019630:1671248-1671517 [Enterobacter asburiae]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCAGAGGTCATCGAGCAGCACCGTCGTC
TCGTATTAAACGAAGCATCATGGACTGCGGTGATGGATGCCATCGACAATCCGCCTGAGCCAAATGAACGCCTGAAACGA
GCAGCAAAACGGCTTCGAAACATGGAGTGA