Detailed information of TA system TA032769

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 603222..603798
• Replicon: chromosome
• Accession: NZ_AP019630
• Organism: Enterobacter asburiae strain 17Nkhm-UP2

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: FOY94_RS02850
• Protein ID: WP_143345958.1
• Coordinates: 603222..603509 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A808I9W7
• Locus tag: FOY94_RS02855
• Protein ID: WP_048980603.1
• Coordinates: 603496..603798 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOY94_RS02825	598323..599018	+	696	WP_048980606.1	winged helix-turn-helix domain-containing protein	-
FOY94_RS02830	599168..599638	+	471	WP_033144545.1	MarR family transcriptional regulator	-
FOY94_RS02835	599635..600702	+	1068	WP_101744430.1	HlyD family secretion protein	-
FOY94_RS02840	600707..601783	+	1077	WP_023310256.1	DUF2955 domain-containing protein	-
FOY94_RS02845	601780..603051	-	1272	WP_048980604.1	DUF445 domain-containing protein	-
FOY94_RS02850	603222..603509	+	288	WP_143345958.1	BrnT family toxin	Toxin
FOY94_RS02855	603496..603798	+	303	WP_048980603.1	BrnA antitoxin family protein	Antitoxin
FOY94_RS02860	603829..604467	-	639	WP_048980602.1	LysE family translocator	-
FOY94_RS02865	604515..605258	-	744	WP_048980600.1	AraC family transcriptional regulator	-
FOY94_RS02870	605410..606780	+	1371	WP_048980598.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
FOY94_RS02875	606826..607146	-	321	WP_039262264.1	hypothetical protein	-
FOY94_RS02880	607146..607691	-	546	WP_023310269.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11247.75 Da
• Isoelectric Point: 6.7559

>T32769 WP_143345958.1 NZ_AP019630:603222-603509 [Enterobacter asburiae]
MPTEFEWDTNKAKSNLIKHGIRFEEAVLVFDDPYHLSLQDRHENGEFRWQTIGLVHGLIVIMVAHTVRFESCDEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T32769 NZ_AP019630:603222-603509 [Enterobacter asburiae]
ATGCCAACGGAGTTTGAATGGGATACCAACAAAGCGAAAAGCAATCTCATTAAGCATGGGATTCGCTTTGAAGAAGCTGT
TCTGGTGTTTGACGATCCTTACCATTTATCTTTGCAGGATCGTCATGAAAATGGAGAGTTCCGTTGGCAGACCATTGGCC
TTGTACATGGTCTAATTGTCATTATGGTCGCACACACGGTACGTTTTGAAAGTTGTGACGAAGTCATTCGAATCATTAGC
GCACGAAAGGCGGACCGTAAAGAGAGGAACCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11451.16 Da
• Isoelectric Point: 10.1522

>AT32769 WP_048980603.1 NZ_AP019630:603496-603798 [Enterobacter asburiae]
MSMVKYKRGSAPEMSARREAELKALAEKSEAEIDYSDIPAMGDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT32769 NZ_AP019630:603496-603798 [Enterobacter asburiae]
ATGAGCATGGTTAAATATAAACGCGGTAGTGCGCCTGAGATGAGTGCTCGCCGTGAGGCTGAGCTCAAAGCACTAGCCGA
AAAATCGGAAGCAGAGATTGATTACAGCGATATCCCTGCCATGGGAGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAGT
TCTATCGGCCACTCAAAACTCAGGCGTCTGTACGTATCGACGCTGACGTGATGGAGTGGCTTAAACGGCCTGGAAAAGGT
TACCAGACGAGGCTCAATGCCATTTTGCGTGAAGCTATGCTGCGCGATCAAAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A808I9W7