32748

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2248443..2248957 Replicon chromosome
Accession NZ_AP019545
Organism Staphylococcus aureus strain KG-22

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag GDK20_RS11435 Protein ID WP_000581792.1
Coordinates 2248443..2248652 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag GDK20_RS11440 Protein ID WP_000138932.1
Coordinates 2248664..2248957 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GDK20_RS11415 2243951..2245309 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
GDK20_RS11420 2245324..2246394 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
GDK20_RS11425 2246712..2247914 + 1203 WP_001109939.1 rod shape-determining protein RodA -
GDK20_RS11430 2248178..2248315 - 138 WP_000828354.1 hypothetical protein -
GDK20_RS11435 2248443..2248652 - 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
GDK20_RS11440 2248664..2248957 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
GDK20_RS11445 2249123..2250607 + 1485 WP_000571549.1 cardiolipin synthase -
GDK20_RS11450 2250635..2251282 + 648 WP_001187630.1 HD domain-containing protein -
GDK20_RS11475 2252195..2253067 - 873 WP_000725802.1 membrane protein insertase YidC -
GDK20_RS11480 2253153..2253794 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T32748 WP_000581792.1 NZ_AP019545:c2248652-2248443 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T32748 NZ_AP019545:c2248652-2248443 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT32748 WP_000138932.1 NZ_AP019545:c2248957-2248664 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT32748 NZ_AP019545:c2248957-2248664 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References