Detailed information of TA system TA032725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2226503..2227032
• Replicon: chromosome
• Accession: NZ_AP019543
• Organism: Staphylococcus aureus strain KG-18

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GDK18_RS11330
• Protein ID: WP_000621175.1
• Coordinates: 2226503..2226865 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: GDK18_RS11335
• Protein ID: WP_000948331.1
• Coordinates: 2226862..2227032 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GDK18_RS11300	2222150..2222920	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
GDK18_RS11305	2222895..2223044	-	150	Protein_2131	ATP-binding protein	-
GDK18_RS11310	2223141..2224313	+	1173	WP_000195429.1	IS256-like element IS256 family transposase	-
GDK18_RS11315	2224365..2224706	-	342	Protein_2133	anti-sigma B factor RsbW	-
GDK18_RS11320	2224708..2225034	-	327	WP_001052491.1	anti-sigma factor antagonist	-
GDK18_RS11325	2225153..2226154	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
GDK18_RS11330	2226503..2226865	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GDK18_RS11335	2226862..2227032	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
GDK18_RS11340	2227117..2228265	-	1149	WP_001281154.1	alanine racemase	-
GDK18_RS11345	2228331..2228690	-	360	WP_000581197.1	holo-ACP synthase	-
GDK18_RS11350	2228694..2229185	-	492	WP_001205912.1	PH domain-containing protein	-
GDK18_RS11355	2229172..2230755	-	1584	WP_001294626.1	PH domain-containing protein	-
GDK18_RS11360	2230748..2231227	-	480	WP_001287087.1	hypothetical protein	-
GDK18_RS11365	2231435..2231995	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2223141..2224313	1172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T32725 WP_000621175.1 NZ_AP019543:c2226865-2226503 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T32725 NZ_AP019543:c2226865-2226503 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT32725 WP_000948331.1 NZ_AP019543:c2227032-2226862 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT32725 NZ_AP019543:c2227032-2226862 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1