Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | kacAT/ElaA-DUF1778 |
| Location | 131966..132769 | Replicon | plasmid pTHO-008_1 |
| Accession | NZ_AP019539 | ||
| Organism | Escherichia coli strain THO-008 | ||
Toxin (Protein)
| Gene name | KacT | Uniprot ID | G3CAI8 |
| Locus tag | QMN35_RS25140 | Protein ID | WP_000348883.1 |
| Coordinates | 132239..132769 (+) | Length | 177 a.a. |
Antitoxin (Protein)
| Gene name | KacA | Uniprot ID | G3CAI7 |
| Locus tag | QMN35_RS25135 | Protein ID | WP_001275013.1 |
| Coordinates | 131966..132235 (+) | Length | 90 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QMN35_RS25110 (ECTHO008_p11450) | 128112..128380 | + | 269 | Protein_163 | type II toxin-antitoxin system ParD family antitoxin | - |
| QMN35_RS25115 | 128367..128656 | + | 290 | Protein_164 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| QMN35_RS25120 | 128723..128905 | + | 183 | WP_042004909.1 | hypothetical protein | - |
| QMN35_RS25125 (ECTHO008_p11470) | 129026..129766 | + | 741 | WP_001586233.1 | tyrosine-type recombinase/integrase | - |
| QMN35_RS25130 (ECTHO008_p11480) | 130089..131078 | - | 990 | WP_123002471.1 | RepB family plasmid replication initiator protein | - |
| QMN35_RS25135 (ECTHO008_p11490) | 131966..132235 | + | 270 | WP_001275013.1 | DUF1778 domain-containing protein | Antitoxin |
| QMN35_RS25140 (ECTHO008_p11500) | 132239..132769 | + | 531 | WP_000348883.1 | GNAT family N-acetyltransferase | Toxin |
| QMN35_RS25145 | 132770..132987 | + | 218 | Protein_170 | transposase | - |
| QMN35_RS25150 (ECTHO008_p11510) | 133043..133359 | - | 317 | Protein_171 | type II toxin-antitoxin system VapC family toxin | - |
| QMN35_RS25155 (ECTHO008_p11520) | 133356..133586 | - | 231 | WP_001261274.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| QMN35_RS25160 | 133543..133641 | + | 99 | Protein_173 | hypothetical protein | - |
| QMN35_RS25165 (ECTHO008_p11530) | 133785..135830 | - | 2046 | WP_251121265.1 | histidinol-phosphatase | - |
| QMN35_RS25170 (ECTHO008_p11540) | 136024..136779 | - | 756 | WP_000065240.1 | IS21-like element ISEc10 family helper ATPase IstB | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27 / mph(A) / sul1 / qacE / aadA5 / dfrA17 | - | 1..151109 | 151109 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 177 a.a. Molecular weight: 20321.20 Da Isoelectric Point: 6.4889
>T32690 WP_000348883.1 NZ_AP019539:132239-132769 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE
Download Length: 531 bp
>T32690 NZ_AP019539:132239-132769 [Escherichia coli]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA
Antitoxin
Download Length: 90 a.a. Molecular weight: 10229.63 Da Isoelectric Point: 6.4702
>AT32690 WP_001275013.1 NZ_AP019539:131966-132235 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE
Download Length: 270 bp
>AT32690 NZ_AP019539:131966-132235 [Escherichia coli]
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|