Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 125257..125816 Replicon plasmid pTHO-008_1
Accession NZ_AP019539
Organism Escherichia coli strain THO-008

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag QMN35_RS25090 Protein ID WP_000421272.1
Coordinates 125541..125816 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag QMN35_RS25085 Protein ID WP_001387467.1
Coordinates 125257..125541 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMN35_RS25060 120720..121160 - 441 Protein_153 zinc-binding dehydrogenase -
QMN35_RS25065 (ECTHO008_p11370) 121561..122451 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
QMN35_RS25070 (ECTHO008_p11380) 122476..122856 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
QMN35_RS25075 (ECTHO008_p11390) 122889..123854 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
QMN35_RS25080 (ECTHO008_p11400) 123900..124652 - 753 WP_000562172.1 IclR family transcriptional regulator -
QMN35_RS25085 (ECTHO008_p11410) 125257..125541 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
QMN35_RS25090 (ECTHO008_p11420) 125541..125816 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QMN35_RS25095 (ECTHO008_p11430) 125922..126125 + 204 Protein_160 hypothetical protein -
QMN35_RS25100 126250..126378 - 129 Protein_161 IS481 family transposase -
QMN35_RS25105 (ECTHO008_p11440) 126411..127580 - 1170 WP_001072355.1 hypothetical protein -
QMN35_RS25110 (ECTHO008_p11450) 128112..128380 + 269 Protein_163 type II toxin-antitoxin system ParD family antitoxin -
QMN35_RS25115 128367..128656 + 290 Protein_164 type II toxin-antitoxin system RelE/ParE family toxin -
QMN35_RS25120 128723..128905 + 183 WP_042004909.1 hypothetical protein -
QMN35_RS25125 (ECTHO008_p11470) 129026..129766 + 741 WP_001586233.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27 / mph(A) / sul1 / qacE / aadA5 / dfrA17 - 1..151109 151109


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T32689 WP_000421272.1 NZ_AP019539:125541-125816 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T32689 NZ_AP019539:125541-125816 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT32689 WP_001387467.1 NZ_AP019539:125257-125541 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT32689 NZ_AP019539:125257-125541 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References