Detailed information of TA system TA032687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4487271..4487866
• Replicon: chromosome
• Accession: NZ_AP019538
• Organism: Escherichia coli strain THO-008

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: QMN35_RS21580
• Protein ID: WP_000239581.1
• Coordinates: 4487271..4487621 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: QMN35_RS21585
• Protein ID: WP_001223213.1
• Coordinates: 4487615..4487866 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMN35_RS21560 (4482718)	4482718..4483740	-	1023	WP_001586590.1	ABC transporter permease	-
QMN35_RS21565 (4483754)	4483754..4485256	-	1503	WP_000210560.1	sugar ABC transporter ATP-binding protein	-
QMN35_RS21570 (4485396)	4485396..4486352	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
QMN35_RS21575 (4486662)	4486662..4487192	+	531	WP_000055075.1	inorganic diphosphatase	-
QMN35_RS21580 (4487271)	4487271..4487621	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
QMN35_RS21585 (4487615)	4487615..4487866	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
QMN35_RS21590 (4488078)	4488078..4488419	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
QMN35_RS21595 (4488422)	4488422..4492201	-	3780	WP_001586589.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T32687 WP_000239581.1 NZ_AP019538:c4487621-4487271 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T32687 NZ_AP019538:c4487621-4487271 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT32687 WP_001223213.1 NZ_AP019538:c4487866-4487615 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT32687 NZ_AP019538:c4487866-4487615 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7