Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4320960..4321536 Replicon chromosome
Accession NZ_AP019538
Organism Escherichia coli strain THO-008

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QMN35_RS20815 Protein ID WP_001297643.1
Coordinates 4321249..4321536 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QMN35_RS20810 Protein ID WP_000063156.1
Coordinates 4320960..4321262 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMN35_RS20795 (4317556) 4317556..4319706 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QMN35_RS20800 (4319756) 4319756..4319959 + 204 WP_000467859.1 YbdD/YjiX family protein -
QMN35_RS20805 (4319970) 4319970..4320926 + 957 WP_001297640.1 GTPase -
QMN35_RS20810 (4320960) 4320960..4321262 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QMN35_RS20815 (4321249) 4321249..4321536 - 288 WP_001297643.1 BrnT family toxin Toxin
QMN35_RS20820 (4321735) 4321735..4322432 - 698 Protein_4067 IS1 family transposase -
QMN35_RS20825 (4322489) 4322489..4322584 + 96 Protein_4068 dienelactone hydrolase family protein -
QMN35_RS20830 (4322730) 4322730..4323962 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
QMN35_RS20835 (4324003) 4324003..4325283 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T32684 WP_001297643.1 NZ_AP019538:c4321536-4321249 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T32684 NZ_AP019538:c4321536-4321249 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT32684 WP_000063156.1 NZ_AP019538:c4321262-4320960 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT32684 NZ_AP019538:c4321262-4320960 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References