Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3736755..3737373 Replicon chromosome
Accession NZ_AP019538
Organism Escherichia coli strain THO-008

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QMN35_RS18045 Protein ID WP_001291435.1
Coordinates 3737155..3737373 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QMN35_RS18040 Protein ID WP_000344800.1
Coordinates 3736755..3737129 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMN35_RS18030 (3731844) 3731844..3733037 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
QMN35_RS18035 (3733060) 3733060..3736209 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
QMN35_RS18040 (3736755) 3736755..3737129 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QMN35_RS18045 (3737155) 3737155..3737373 + 219 WP_001291435.1 HHA domain-containing protein Toxin
QMN35_RS18050 (3737545) 3737545..3738096 + 552 WP_000102564.1 maltose O-acetyltransferase -
QMN35_RS18055 (3738212) 3738212..3738682 + 471 WP_000136192.1 YlaC family protein -
QMN35_RS18060 (3738846) 3738846..3740396 + 1551 WP_001305493.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QMN35_RS18065 (3740438) 3740438..3740791 - 354 WP_000878140.1 DUF1428 domain-containing protein -
QMN35_RS18075 (3741170) 3741170..3741481 + 312 WP_000409911.1 MGMT family protein -
QMN35_RS18080 (3741512) 3741512..3742084 - 573 WP_000779833.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T32682 WP_001291435.1 NZ_AP019538:3737155-3737373 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T32682 NZ_AP019538:3737155-3737373 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT32682 WP_000344800.1 NZ_AP019538:3736755-3737129 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT32682 NZ_AP019538:3736755-3737129 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References