Detailed information of TA system TA032681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3282099..3282804
• Replicon: chromosome
• Accession: NZ_AP019538
• Organism: Escherichia coli strain THO-008

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: QMN35_RS15900
• Protein ID: WP_000539521.1
• Coordinates: 3282099..3282485 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: QMN35_RS15905
• Protein ID: WP_001280945.1
• Coordinates: 3282475..3282804 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMN35_RS15880 (3278103)	3278103..3278729	+	627	WP_001314584.1	glutathione S-transferase GstB	-
QMN35_RS15885 (3278726)	3278726..3279841	-	1116	WP_000555033.1	aldose sugar dehydrogenase YliI	-
QMN35_RS15890 (3279952)	3279952..3280335	-	384	WP_000497137.1	biofilm formation regulator BssR	-
QMN35_RS15895 (3280548)	3280548..3281873	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
QMN35_RS15900 (3282099)	3282099..3282485	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QMN35_RS15905 (3282475)	3282475..3282804	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
QMN35_RS15910 (3282874)	3282874..3284202	-	1329	WP_001586846.1	GGDEF domain-containing protein	-
QMN35_RS15915 (3284210)	3284210..3286558	-	2349	WP_001305945.1	EAL domain-containing protein	-
QMN35_RS15920 (3286736)	3286736..3287647	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T32681 WP_000539521.1 NZ_AP019538:3282099-3282485 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T32681 NZ_AP019538:3282099-3282485 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT32681 WP_001280945.1 NZ_AP019538:3282475-3282804 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT32681 NZ_AP019538:3282475-3282804 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6