Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 997002..997656 Replicon chromosome
Accession NZ_AP019538
Organism Escherichia coli strain THO-008

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag QMN35_RS04825 Protein ID WP_000244781.1
Coordinates 997249..997656 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QMN35_RS04820 Protein ID WP_000354046.1
Coordinates 997002..997268 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMN35_RS04800 (993090) 993090..994523 - 1434 WP_001587402.1 6-phospho-beta-glucosidase BglA -
QMN35_RS04805 (994568) 994568..994879 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
QMN35_RS04810 (995043) 995043..995702 + 660 WP_000250274.1 hemolysin III family protein -
QMN35_RS04815 (995779) 995779..996759 - 981 WP_001587401.1 tRNA-modifying protein YgfZ -
QMN35_RS04820 (997002) 997002..997268 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QMN35_RS04825 (997249) 997249..997656 + 408 WP_000244781.1 protein YgfX Toxin
QMN35_RS04830 (997696) 997696..998217 - 522 WP_001055874.1 flavodoxin FldB -
QMN35_RS04835 (998329) 998329..999225 + 897 WP_001587400.1 site-specific tyrosine recombinase XerD -
QMN35_RS04840 (999250) 999250..999960 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QMN35_RS04845 (999966) 999966..1001699 + 1734 WP_001587399.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T32667 WP_000244781.1 NZ_AP019538:997249-997656 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T32667 NZ_AP019538:997249-997656 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT32667 WP_000354046.1 NZ_AP019538:997002-997268 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT32667 NZ_AP019538:997002-997268 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References