Detailed information of TA system TA032660

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 2225363..2226129
• Replicon: chromosome
• Accession: NZ_AP019531
• Organism: Serratia symbiotica strain IS

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: SSYIS1_RS11290
• Protein ID: WP_149590852.1
• Coordinates: 2225363..2225731 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: E9CM08
• Locus tag: SSYIS1_RS11295
• Protein ID: WP_006708729.1
• Coordinates: 2225794..2226129 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SSYIS1_RS11255	2220826..2221893	-	1068	WP_172624196.1	ATP-binding cassette domain-containing protein	-
SSYIS1_RS11260	2221856..2222263	-	408	WP_071985770.1	ATP-binding cassette domain-containing protein	-
SSYIS1_RS11265	2222457..2223683	-	1227	WP_149590851.1	MFS transporter	-
SSYIS1_RS11270	2223680..2223919	-	240	WP_037388646.1	alpha/beta hydrolase	-
SSYIS1_RS11275	2224036..2224194	-	159	Protein_2154	hydroxyacid dehydrogenase	-
SSYIS1_RS11280	2224185..2224310	-	126	Protein_2155	hydroxyacid dehydrogenase	-
SSYIS1_RS11285	2224397..2225230	-	834	WP_006708727.1	DUF4942 domain-containing protein	-
SSYIS1_RS11290	2225363..2225731	-	369	WP_149590852.1	TA system toxin CbtA family protein	Toxin
SSYIS1_RS11295	2225794..2226129	-	336	WP_006708729.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
SSYIS1_RS11300	2226141..2226620	-	480	WP_006708730.1	DNA repair protein RadC	-
SSYIS1_RS11305	2226803..2227423	-	621	WP_006708731.1	hypothetical protein	-
SSYIS1_RS11310	2227451..2228006	-	556	Protein_2161	hypothetical protein	-
SSYIS1_RS11320	2228525..2229719	+	1195	Protein_2162	IS91 family transposase	-
SSYIS1_RS11325	2229876..2230283	+	408	Protein_2163	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13773.72 Da
• Isoelectric Point: 5.6133

>T32660 WP_149590852.1 NZ_AP019531:c2225731-2225363 [Serratia symbiotica]
MQTLPVTPKRAAYAYPSPVVVWQSMLTYLLEQHYGLALNDTEFSDDAVIHEHIDAGISLADALNFTVEKFDLVRIDRRGF
SCQEQSPFITAIDILRARRATGLMTRLDYQAITSVIRGEKQA

Nucleotide

• Length: 369 bp

>T32660 NZ_AP019531:c2225731-2225363 [Serratia symbiotica]
ATGCAGACCTTACCTGTCACCCCGAAACGGGCGGCTTACGCTTACCCATCACCCGTTGTTGTCTGGCAATCCATGCTCAC
GTATCTGCTGGAACAGCATTACGGATTGGCGCTTAACGATACCGAGTTCAGTGATGATGCTGTGATCCACGAACATATTG
ATGCCGGTATCTCTCTGGCTGATGCGTTGAATTTCACGGTGGAAAAGTTCGACCTGGTTCGCATCGATCGCCGGGGGTTC
TCCTGCCAGGAACAATCTCCCTTCATCACGGCTATCGATATTCTCCGTGCCCGTCGAGCCACTGGCTTGATGACCCGTCT
GGATTACCAGGCCATCACCTCAGTTATCCGGGGAGAAAAGCAGGCATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12327.92 Da
• Isoelectric Point: 5.9498

>AT32660 WP_006708729.1 NZ_AP019531:c2226129-2225794 [Serratia symbiotica]
MPSITTPAWGLKRNVTPQFGARLVQEGDRLHFLADRADISGTFSEAQVRDLNNAFPEFINHLELMLLSGELNPLHQHCVT
LYRNGLTCEADSLGSHGYVYLAIYPTPQSTA

Nucleotide

• Length: 336 bp

>AT32660 NZ_AP019531:c2226129-2225794 [Serratia symbiotica]
ATGCCATCAATCACTACCCCAGCCTGGGGACTTAAACGCAACGTCACTCCGCAGTTTGGTGCCCGCCTGGTACAAGAAGG
CGACCGGCTGCATTTTCTGGCCGACCGTGCCGATATCAGCGGGACATTCAGCGAAGCACAAGTACGTGATTTAAATAACG
CCTTCCCGGAGTTTATCAACCACCTGGAACTGATGCTGCTCTCCGGTGAGCTCAACCCTCTCCACCAGCACTGTGTCACG
TTGTATCGCAATGGCTTGACCTGTGAGGCCGATAGCCTGGGATCACACGGTTATGTGTATCTCGCCATTTATCCTACTCC
CCAATCAACTGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A455VJ82