Detailed information of TA system TA032654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2492270..2492918
• Replicon: chromosome
• Accession: NZ_AP019516
• Organism: Psychrobacter sp. KH172YL61

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: PKHYL_RS13300
• Protein ID: WP_240365371.1
• Coordinates: 2492270..2492551 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1D9D1W8
• Locus tag: PKHYL_RS13305
• Protein ID: WP_071003129.1
• Coordinates: 2492571..2492918 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PKHYL_RS13275 (PKHYL_32450)	2487589..2489072	+	1484	Protein_2692	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA	-
PKHYL_RS13280 (PKHYL_32460)	2489085..2490614	+	1530	Protein_2693	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
PKHYL_RS13295 (PKHYL_32480)	2490969..2492210	+	1242	WP_071003131.1	integrase arm-type DNA-binding domain-containing protein	-
PKHYL_RS13300 (PKHYL_32490)	2492270..2492551	+	282	WP_240365371.1	type II toxin-antitoxin system HicA family toxin	Toxin
PKHYL_RS13305 (PKHYL_32500)	2492571..2492918	+	348	WP_071003129.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PKHYL_RS13310 (PKHYL_32510)	2493027..2493647	+	621	WP_071003128.1	hypothetical protein	-
PKHYL_RS13315 (PKHYL_32520)	2493653..2494072	+	420	WP_240365372.1	transposase family protein	-
PKHYL_RS13320 (PKHYL_32530)	2494032..2494484	+	453	WP_240365373.1	transposase family protein	-
PKHYL_RS13325 (PKHYL_32540)	2494481..2495155	+	675	WP_240365374.1	hypothetical protein	-
PKHYL_RS13330 (PKHYL_32550)	2495567..2496007	-	441	WP_071003125.1	hypothetical protein	-
PKHYL_RS13335 (PKHYL_32560)	2496096..2496377	-	282	WP_071003121.1	DUF503 domain-containing protein	-
PKHYL_RS13340 (PKHYL_32570)	2496496..2497284	+	789	WP_025645975.1	threonine/serine exporter family protein	-
PKHYL_RS17750	2497342..2497515	+	174	WP_269778319.1	hypothetical protein	-
PKHYL_RS17755 (PKHYL_32580)	2497544..2497732	+	189	WP_269778179.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-75)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10276.79 Da
• Isoelectric Point: 8.5815

>T32654 WP_240365371.1 NZ_AP019516:2492270-2492551 [Psychrobacter sp. KH172YL61]
MQRKHLKTLNIIFTKPISANIKWADIEALFIALGAEVNERAGSRIAVVLDGEVQVYHRPHPQPTTDKGAVASVRKWLDSL
GYSPIDEAKDTAP

Nucleotide

• Length: 282 bp

>T32654 NZ_AP019516:2492270-2492551 [Psychrobacter sp. KH172YL61]
ATGCAGCGCAAGCACCTAAAAACACTTAATATCATCTTTACTAAGCCTATATCAGCAAATATAAAGTGGGCAGACATTGA
AGCCTTATTTATAGCATTAGGTGCAGAGGTCAACGAACGGGCAGGATCAAGAATAGCTGTAGTGTTAGATGGTGAGGTAC
AAGTTTATCACCGACCACACCCCCAACCGACAACAGATAAGGGCGCAGTCGCTAGCGTTCGTAAATGGCTAGATAGTTTA
GGGTATAGCCCTATTGATGAAGCCAAAGACACCGCCCCGTAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 12730.30 Da
• Isoelectric Point: 4.3422

>AT32654 WP_071003129.1 NZ_AP019516:2492571-2492918 [Psychrobacter sp. KH172YL61]
MQNIMMINDHKALIQYDPELGLFRGEFVGLNGGADFYGDSIADLENEGKQSLQTFLDVCQEQGIEPYKSFSGKFVTRVPS
ELHEQVTTIATSHGMSLNQWVTNTLANAIKTEGMA

Nucleotide

• Length: 348 bp

>AT32654 NZ_AP019516:2492571-2492918 [Psychrobacter sp. KH172YL61]
ATGCAAAACATTATGATGATTAATGACCATAAAGCACTTATTCAATATGACCCTGAACTAGGGTTATTCCGTGGAGAATT
TGTAGGGCTAAATGGTGGGGCAGACTTTTATGGTGACAGCATTGCCGATTTAGAAAATGAGGGTAAGCAGTCATTGCAAA
CCTTTTTGGACGTTTGCCAAGAACAAGGCATTGAGCCTTACAAATCCTTTTCAGGTAAGTTTGTAACCCGTGTGCCAAGT
GAGCTACACGAGCAAGTAACAACTATTGCCACCAGCCACGGTATGAGCCTTAACCAATGGGTCACTAATACACTTGCCAA
TGCCATCAAAACCGAAGGCATGGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A1D9D1W8