32652

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 5379772..5380313 Replicon chromosome
Accession NZ_AP019411
Organism Pseudomonas syringae pv. actinidiae strain MAFF613020

Toxin (Protein)


Gene name relE Uniprot ID A0A2V0Q684
Locus tag M2494_RS24000 Protein ID WP_003382250.1
Coordinates 5379772..5380047 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0K8LU58
Locus tag M2494_RS24005 Protein ID WP_003382249.1
Coordinates 5380047..5380313 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2494_RS23980 (KPSA1B_104883) 5374903..5375928 - 1026 WP_003380375.1 TRAP transporter substrate-binding protein -
M2494_RS23985 (KPSA1B_104884) 5375985..5376869 - 885 WP_003380378.1 SMP-30/gluconolactonase/LRE family protein -
M2494_RS23990 (KPSA1B_104885) 5376929..5377753 - 825 WP_003380380.1 NAD(P)-dependent oxidoreductase -
M2494_RS23995 (KPSA1B_104886) 5377932..5379200 - 1269 WP_017698468.1 OprD family porin -
M2494_RS24000 (KPSA1B_104887) 5379772..5380047 - 276 WP_003382250.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M2494_RS24005 (KPSA1B_104888) 5380047..5380313 - 267 WP_003382249.1 ribbon-helix-helix domain-containing protein Antitoxin
M2494_RS24010 (KPSA1B_104889) 5380478..5382400 - 1923 WP_017698469.1 methyl-accepting chemotaxis protein -
M2494_RS24015 (KPSA1B_104890) 5382759..5384639 + 1881 WP_003382247.1 methyl-accepting chemotaxis protein -
M2494_RS24020 (KPSA1B_104891) 5384767..5385030 - 264 WP_017683360.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5370371..5380313 9942


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(8-45)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10720.38 Da        Isoelectric Point: 7.0123

>T32652 WP_003382250.1 NZ_AP019411:c5380047-5379772 [Pseudomonas syringae pv. actinidiae]
MELMWTSKALSDVARLYDFLAVANQPAAAQTVQKLTAAPIILLSNPRIGERLEEFEPRDVRRIQIGRYEMRYEIVDSTIY
LLRLWHTREDR

Download         Length: 276 bp

>T32652 NZ_AP019411:c5380047-5379772 [Pseudomonas syringae pv. actinidiae]
ATGGAGCTGATGTGGACAAGCAAAGCGCTATCTGACGTCGCCCGCCTGTATGATTTTTTAGCGGTAGCAAACCAGCCCGC
TGCCGCCCAGACCGTACAGAAGCTTACAGCGGCGCCGATCATACTCCTTTCCAACCCGCGGATTGGTGAGCGTCTGGAGG
AGTTTGAGCCCCGGGATGTGCGCAGAATTCAGATAGGCCGCTATGAAATGCGCTACGAAATTGTGGACTCAACAATTTAT
CTGTTGCGGCTGTGGCATACACGCGAGGATCGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9934.24 Da        Isoelectric Point: 5.7066

>AT32652 WP_003382249.1 NZ_AP019411:c5380313-5380047 [Pseudomonas syringae pv. actinidiae]
MTTKTRSVTAHIPEQLAEKVDLMAERLERSKNWIVKQALSAWIDQEEERSRLTREALADVDAGRVIDHQAVQAWADSLST
ATPLPVPR

Download         Length: 267 bp

>AT32652 NZ_AP019411:c5380313-5380047 [Pseudomonas syringae pv. actinidiae]
ATGACAACCAAGACCCGATCTGTAACAGCTCACATACCCGAACAATTGGCTGAAAAAGTCGACCTGATGGCAGAGCGACT
CGAACGCTCTAAAAACTGGATCGTCAAACAGGCCTTGTCTGCATGGATAGATCAGGAGGAAGAACGTAGTCGCCTCACTC
GCGAAGCATTAGCCGATGTCGATGCTGGCCGTGTGATTGACCATCAGGCTGTTCAGGCCTGGGCTGACAGTTTAAGCACC
GCTACGCCTCTGCCGGTTCCGCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0Q684

Antitoxin

Source ID Structure
AlphaFold DB A0A0K8LU58

References