32649

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 3979222..3980951 Replicon chromosome
Accession NZ_AP019411
Organism Pseudomonas syringae pv. actinidiae strain MAFF613020

Toxin (Protein)


Gene name pmenT Uniprot ID A0A2V0QPP4
Locus tag M2494_RS17745 Protein ID WP_003379114.1
Coordinates 3979222..3979866 (+) Length 215 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A2V0QNZ0
Locus tag M2494_RS17750 Protein ID WP_003379115.1
Coordinates 3979863..3980951 (+) Length 363 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2494_RS17715 (KPSA1B_103618) 3974612..3974866 + 255 WP_003377256.1 hypothetical protein -
M2494_RS17720 3974785..3974955 - 171 Protein_3493 DUF4411 domain-containing protein -
M2494_RS17725 (KPSA1B_103619) 3974968..3975642 - 675 WP_003377258.1 HAD family hydrolase -
M2494_RS17730 3975835..3977012 + 1178 WP_164706461.1 IS3 family transposase -
M2494_RS17735 (KPSA1B_103622) 3977105..3978226 - 1122 WP_003379112.1 endonuclease NucS -
M2494_RS17740 (KPSA1B_103623) 3978644..3979225 + 582 WP_003379113.1 DarT ssDNA thymidine ADP-ribosyltransferase family protein -
M2494_RS17745 (KPSA1B_103624) 3979222..3979866 + 645 WP_003379114.1 DUF4433 domain-containing protein Toxin
M2494_RS17750 (KPSA1B_103625) 3979863..3980951 + 1089 WP_003379115.1 macro domain-containing protein Antitoxin
M2494_RS29820 (KPSA1B_103627) 3981158..3981304 - 147 WP_017699797.1 hypothetical protein -
M2494_RS17760 (KPSA1B_103628) 3981385..3981900 - 516 Protein_3501 ABC transporter ATP-binding protein -
M2494_RS17765 (KPSA1B_103629) 3981982..3982737 - 756 WP_017699032.1 IS21-like element ISPsy14 family helper ATPase IstB -
M2494_RS17770 (KPSA1B_103630) 3982758..3984272 - 1515 WP_077142728.1 IS21-like element ISPsy41 family transposase -
M2494_RS17775 (KPSA1B_103631) 3984404..3984604 - 201 Protein_3504 hypothetical protein -
M2494_RS17780 (KPSA1B_103632) 3984601..3985377 - 777 WP_003379118.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3975835..4003729 27894
- inside IScluster/Tn - - 3975835..3984272 8437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-213)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 215 a.a.        Molecular weight: 24813.38 Da        Isoelectric Point: 6.4920

>T32649 WP_003379114.1 NZ_AP019411:3979222-3979866 [Pseudomonas syringae pv. actinidiae]
MIYSSLNPEKALIWRIVHRDNLPWILDNGLHCASSYMQAPQYVNIGNADLIDKRRNRSVPIAPAGVLSDYVPFYFTPFSV
MMKNIHSGWSVQQRSNDEIVILVSSLPRIEQLGLAFVFTNAHAYPDWTDYYSDLVNLDQIDWPIIQKRDFKRDPDDPRKM
ERYQAEALIHHHLPITGLLGIMCYDDAMKERIEQDVAARGLTLPVHARPGWYFQ

Download         Length: 645 bp

>T32649 NZ_AP019411:3979222-3979866 [Pseudomonas syringae pv. actinidiae]
ATGATCTACAGCAGTCTGAATCCAGAAAAAGCTCTGATTTGGCGAATCGTCCACCGCGATAATCTGCCTTGGATTCTGGA
CAATGGCCTGCATTGCGCAAGCTCCTACATGCAAGCACCGCAGTACGTGAACATCGGCAACGCTGACCTGATCGACAAAC
GCCGCAATCGCAGTGTTCCAATTGCTCCAGCGGGCGTACTGTCCGACTACGTGCCCTTCTACTTCACACCTTTCTCTGTG
ATGATGAAAAATATCCATTCCGGCTGGAGCGTTCAGCAGCGTAGCAATGACGAGATAGTGATTCTGGTCTCAAGCCTGCC
TCGTATTGAGCAGCTTGGCCTGGCGTTCGTCTTTACCAACGCACACGCCTACCCTGACTGGACCGATTACTACAGCGATC
TGGTGAATCTCGACCAGATCGACTGGCCTATAATCCAGAAACGAGACTTCAAGCGTGACCCTGATGATCCGCGTAAGATG
GAGCGTTATCAGGCCGAAGCGCTCATTCACCATCACCTACCGATTACAGGACTCCTGGGCATCATGTGCTACGACGATGC
AATGAAAGAACGCATTGAGCAAGACGTTGCCGCCCGAGGTCTGACTTTGCCTGTCCACGCGCGTCCGGGATGGTATTTCC
AATGA

Antitoxin


Download         Length: 363 a.a.        Molecular weight: 40646.66 Da        Isoelectric Point: 6.1829

>AT32649 WP_003379115.1 NZ_AP019411:3979863-3980951 [Pseudomonas syringae pv. actinidiae]
MIRFTQGNLLEADTEALVNTVNTVGVMGKGIALMFKERFTENYRLYSAACKAGEVETGKVHVTAVNELDGPRWIVNFPTK
RHWRSPSQMAWITEGLRDLRRFLIDNHVKSVAVPPLGAGNGGLKWVEVREQIVDVLSDLDVDVLVFEPTDQYLNVAKRSG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERSIEQLPSAKNPLDLKFVAHKYGPYANRLEHLLDNLDGSYLH
CDKRISDAGIDDVIWFDEGRKAFLQTYLKTEAKEYSQALERTAELIDGFESPFGMELLATVDWLLCKSGVSPTVPTVREA
LKHWDGGAGAAARKSRLFDDKAIDIALKRLTTSSLSPEMPPS

Download         Length: 1089 bp

>AT32649 NZ_AP019411:3979863-3980951 [Pseudomonas syringae pv. actinidiae]
ATGATCAGATTCACCCAAGGCAATCTGCTGGAAGCAGATACCGAAGCTCTCGTCAACACGGTGAACACTGTAGGCGTAAT
GGGAAAAGGCATTGCGCTGATGTTCAAGGAGCGCTTTACCGAAAACTATCGCCTCTATTCTGCGGCGTGCAAGGCGGGTG
AGGTAGAGACCGGAAAGGTTCACGTAACGGCAGTCAACGAGCTGGACGGCCCGCGCTGGATAGTGAATTTCCCTACCAAG
CGTCACTGGCGTTCCCCCTCGCAGATGGCATGGATCACCGAAGGCTTACGGGATCTGCGCCGTTTTTTGATCGATAACCA
CGTGAAGTCCGTAGCGGTTCCACCTCTCGGGGCGGGTAATGGCGGTTTGAAATGGGTAGAGGTCCGTGAGCAGATTGTTG
ATGTCTTGAGTGATCTGGATGTCGATGTACTTGTCTTTGAACCCACTGATCAATATCTGAATGTCGCGAAGCGTAGCGGT
GTCGAGAAACTGACGCCTGCACGCGCACTCATTGCCGAACTCGTTCGGCGTTACTGGGTTTTGGGCATGGAATGCAGCCT
GCTTGAGATTCAGAAGCTCGCTTGGTTTCTGGAGCGTTCGATAGAGCAATTGCCAAGCGCCAAGAACCCCTTGGATCTCA
AATTTGTAGCCCACAAATATGGGCCATACGCCAATCGCCTTGAGCATCTGCTCGACAATCTCGATGGTAGCTACCTGCAT
TGCGATAAACGCATCAGCGACGCAGGTATTGACGACGTGATCTGGTTTGACGAGGGCCGTAAGGCTTTTCTGCAAACTTA
TCTGAAAACAGAGGCCAAGGAATATTCCCAGGCTCTGGAGCGCACCGCAGAGTTGATTGACGGCTTTGAGTCGCCTTTCG
GCATGGAACTGTTGGCGACGGTTGACTGGTTATTGTGTAAGTCGGGAGTGTCTCCAACGGTTCCGACAGTGCGTGAAGCA
TTGAAGCATTGGGATGGAGGCGCAGGTGCGGCAGCTCGGAAAAGCAGGTTGTTTGATGATAAAGCGATCGACATTGCTCT
AAAGCGATTGACTACCAGTAGTCTCAGCCCAGAGATGCCCCCTTCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0QPP4

Antitoxin

Source ID Structure
AlphaFold DB A0A2V0QNZ0

References