32647

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 2384984..2385603 Replicon chromosome
Accession NZ_AP019411
Organism Pseudomonas syringae pv. actinidiae strain MAFF613020

Toxin (Protein)


Gene name tad Uniprot ID A0A2G9L4Q9
Locus tag M2494_RS10315 Protein ID WP_004397564.1
Coordinates 2385301..2385603 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID A0A0K8LSV9
Locus tag M2494_RS10310 Protein ID WP_004397562.1
Coordinates 2384984..2385304 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2494_RS10295 (KPSA1B_102103) 2380148..2381482 + 1335 WP_004397555.1 AcvB/VirJ family lysyl-phosphatidylglycerol hydrolase -
M2494_RS10300 (KPSA1B_102104) 2381577..2383472 - 1896 WP_004397556.1 potassium transporter Kup -
M2494_RS10305 (KPSA1B_102105) 2383794..2384882 + 1089 WP_004397558.1 catalase family protein -
M2494_RS10310 (KPSA1B_102106) 2384984..2385304 - 321 WP_004397562.1 helix-turn-helix transcriptional regulator Antitoxin
M2494_RS10315 (KPSA1B_102107) 2385301..2385603 - 303 WP_004397564.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M2494_RS10320 (KPSA1B_102108) 2385890..2387233 + 1344 WP_004397566.1 30S ribosomal protein S12 methylthiotransferase RimO -
M2494_RS10325 (KPSA1B_102109) 2387335..2387775 + 441 WP_004397568.1 GNAT family N-acetyltransferase -
M2494_RS10330 (KPSA1B_102110) 2387869..2388579 + 711 WP_004397570.1 rRNA pseudouridine synthase -
M2494_RS10335 (KPSA1B_102111) 2388661..2389122 + 462 WP_004397572.1 DUF1456 family protein -
M2494_RS10340 (KPSA1B_102112) 2389136..2389828 + 693 WP_004397574.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(1-89)

Antitoxin

HigA2-like, helix-turn-helix domain

(11-90)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11152.69 Da        Isoelectric Point: 8.0629

>T32647 WP_004397564.1 NZ_AP019411:c2385603-2385301 [Pseudomonas syringae pv. actinidiae]
MPDEVQDVFGFALSLAQEGSKHSQAKPMKGFSGAGVLEVVEDYDSDTYRAVYSVKFDAMVYVLHCFQKKSTSGIKTAKHD
IELIKSRLKAVQAHVESNRR

Download         Length: 303 bp

>T32647 NZ_AP019411:c2385603-2385301 [Pseudomonas syringae pv. actinidiae]
ATGCCTGACGAGGTGCAGGATGTATTTGGATTCGCCCTGAGTCTCGCGCAGGAAGGCTCCAAACACTCTCAAGCCAAACC
GATGAAGGGTTTCTCCGGAGCAGGGGTGTTGGAGGTCGTTGAGGACTATGATAGTGACACCTATCGCGCTGTATATTCCG
TGAAATTCGATGCAATGGTCTACGTTCTGCACTGTTTTCAGAAAAAGTCGACCTCGGGCATTAAAACGGCAAAACATGAT
ATTGAATTGATCAAATCACGACTCAAAGCCGTACAAGCACATGTAGAGAGTAATAGACGATGA

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11639.62 Da        Isoelectric Point: 10.2027

>AT32647 WP_004397562.1 NZ_AP019411:c2385304-2384984 [Pseudomonas syringae pv. actinidiae]
MIDIEEGSGNVYADLGRADANEMSIKAQLASKIGTIIKARHLTQLQAAEILAMPQPKVSEMLRGKFRGISEAKMMDCLSR
LGRDVQIVVKAAPRSRREGRVEVVFA

Download         Length: 321 bp

>AT32647 NZ_AP019411:c2385304-2384984 [Pseudomonas syringae pv. actinidiae]
ATGATTGATATCGAAGAAGGTAGCGGCAACGTTTACGCTGATCTTGGGCGAGCTGATGCGAACGAAATGTCAATCAAGGC
TCAATTAGCCTCAAAAATCGGCACAATCATCAAGGCTCGACACCTGACCCAGCTCCAGGCGGCTGAGATACTGGCAATGC
CTCAGCCAAAGGTTTCAGAGATGCTTAGGGGCAAGTTCAGAGGCATAAGCGAGGCCAAAATGATGGACTGCCTTTCGCGT
CTCGGACGTGACGTGCAGATTGTGGTCAAGGCAGCACCTCGTTCTCGGCGAGAGGGGCGAGTTGAAGTTGTATTTGCTTA
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G9L4Q9

Antitoxin

Source ID Structure
AlphaFold DB A0A0K8LSV9

References