Detailed information of TA system TA032635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 781554..782075
• Replicon: chromosome
• Accession: NZ_AP019372
• Organism: Staphylococcus pseudintermedius strain SP79

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: GSP_RS03485
• Protein ID: WP_110178839.1
• Coordinates: 781854..782075 (+)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A166R1D0
• Locus tag: GSP_RS03480
• Protein ID: WP_014613363.1
• Coordinates: 781554..781841 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSP_RS03455	776815..777618	+	804	WP_014613358.1	hydroxyethylthiazole kinase	-
GSP_RS03460	777608..778246	+	639	WP_014613359.1	thiamine phosphate synthase	-
GSP_RS03465	778335..779201	+	867	WP_014613360.1	membrane protein insertase YidC	-
GSP_RS03470	779255..779911	-	657	WP_063279128.1	HD domain-containing protein	-
GSP_RS03475	779932..781422	-	1491	WP_127505007.1	cardiolipin synthase	-
GSP_RS03480	781554..781841	+	288	WP_014613363.1	copper-sensing transcriptional repressor CsoR	Antitoxin
GSP_RS03485	781854..782075	+	222	WP_110178839.1	heavy-metal-associated domain-containing protein	Toxin
GSP_RS03490	782079..782285	+	207	WP_014613365.1	cation transporter	-
GSP_RS03495	782386..784566	+	2181	WP_127505009.1	heavy metal translocating P-type ATPase	-
GSP_RS11875	784652..784798	+	147	WP_014613367.1	hypothetical protein	-
GSP_RS03500	785916..786644	+	729	WP_063279125.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (21-64)

Antitoxin

• Metal-sensitive transcriptional repressor (3-93)

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8081.10 Da
• Isoelectric Point: 4.3480

>T32635 WP_110178839.1 NZ_AP019372:781854-782075 [Staphylococcus pseudintermedius]
MENGYVKVTQLTTEAQQQQLSDRLLRLIGVESVAIDVATQSLTLTYDTPANLNTIEKEIYDAGYPVIQSHKGV

Nucleotide

• Length: 222 bp

>T32635 NZ_AP019372:781854-782075 [Staphylococcus pseudintermedius]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGTCTGTTGCTATTGATGTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATATCCTGTTATCCAATCACATAAAGGAGTGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10997.59 Da
• Isoelectric Point: 7.0504

>AT32635 WP_014613363.1 NZ_AP019372:781554-781841 [Staphylococcus pseudintermedius]
MTEQAHHSEEIKRNLKSRLNRIEGQVKAINRMIDEDVYCDDVLTQIRATRSALNSVASRLLDHHMKGCIKHKIDAGHQDE
AMEELLVTFQKLMKD

Nucleotide

• Length: 288 bp

>AT32635 NZ_AP019372:781554-781841 [Staphylococcus pseudintermedius]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAA
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGATGCATCAAGCATAAGATTGACGCGGGACACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACATTTCAAAAACTCATGAAAGATTAG

Structures

Antitoxin

• AlphaFold DB: A0A166R1D0