Detailed information of TA system TA032630

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4131776..4132272
• Replicon: chromosome
• Accession: NZ_AP019314
• Organism: Microcystis viridis NIES-102

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: I4IHG7
• Locus tag: myaer102_RS21230
• Protein ID: WP_002768607.1
• Coordinates: 4132084..4132272 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: I4IHG6
• Locus tag: myaer102_RS21225
• Protein ID: WP_002763050.1
• Coordinates: 4131776..4132072 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
myaer102_RS21190	4126982..4127275	-	294	WP_125732861.1	hypothetical protein	-
myaer102_RS21195	4127374..4128528	+	1155	WP_012265305.1	ISL3 family transposase	-
myaer102_RS21200	4129065..4130001	-	937	Protein_3959	IS110-like element ISMae40 family transposase	-
myaer102_RS30900	4130007..4130150	+	144	WP_164516978.1	hypothetical protein	-
myaer102_RS21210	4130426..4130968	-	543	WP_079205648.1	transposase	-
myaer102_RS21225	4131776..4132072	-	297	WP_002763050.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
myaer102_RS21230	4132084..4132272	-	189	WP_002768607.1	type II toxin-antitoxin system HicA family toxin	Toxin
myaer102_RS30905	4132290..4132451	-	162	WP_164516999.1	hypothetical protein	-
myaer102_RS21235	4132658..4133594	-	937	Protein_3965	IS110-like element ISMae40 family transposase	-
myaer102_RS30910	4133600..4133743	+	144	WP_164516978.1	hypothetical protein	-
myaer102_RS21245	4134174..4137200	-	3027	WP_125731525.1	serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	4127374..4133594	6220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-91)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7161.44 Da
• Isoelectric Point: 10.8174

>T32630 WP_002768607.1 NZ_AP019314:c4132272-4132084 [Microcystis viridis NIES-102]
MESREIIKKLKDEGWYQVGTTGSHHHFKHPHKKGKVTVPHPKKDIPLKTLISIEKQAGIKLR

Nucleotide

• Length: 189 bp

>T32630 NZ_AP019314:c4132272-4132084 [Microcystis viridis NIES-102]
ATGGAAAGTCGAGAAATTATCAAAAAACTGAAGGATGAGGGATGGTATCAAGTAGGTACAACAGGAAGTCATCATCATTT
TAAACATCCCCATAAAAAAGGGAAAGTGACTGTTCCTCACCCTAAAAAGGATATTCCCCTTAAAACCCTAATCAGTATCG
AAAAACAAGCCGGTATTAAATTACGATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10797.16 Da
• Isoelectric Point: 4.1825

>AT32630 WP_002763050.1 NZ_AP019314:c4132072-4131776 [Microcystis viridis NIES-102]
MITYLATVHKDNHSDYGVQFYDFPGCISAGETIEEAKKMATEALKGHISFMLADGDEIPTPSTLETILTDADHQDAIAFL
LIEVSEAILNHQELCVKN

Nucleotide

• Length: 297 bp

>AT32630 NZ_AP019314:c4132072-4131776 [Microcystis viridis NIES-102]
ATGATTACTTATTTAGCCACCGTTCATAAAGATAACCATAGTGATTATGGGGTACAATTTTATGACTTTCCGGGTTGTAT
TAGTGCAGGAGAAACCATCGAAGAAGCGAAAAAAATGGCAACTGAAGCCTTAAAAGGTCATATTTCCTTCATGTTGGCAG
ATGGGGACGAAATTCCCACCCCTAGCACGCTCGAAACCATTTTAACCGACGCGGATCATCAAGATGCGATCGCTTTTCTG
CTCATTGAGGTATCAGAAGCTATTTTAAACCATCAGGAATTGTGTGTAAAAAACTAG

Structures

Toxin

• AlphaFold DB: I4IHG7

Antitoxin

• AlphaFold DB: I4IHG6