Detailed information of TA system TA032628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2209073..2209663
• Replicon: chromosome
• Accession: NZ_AP019314
• Organism: Microcystis viridis NIES-102

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: I4ID82
• Locus tag: myaer102_RS11370
• Protein ID: WP_008201144.1
• Coordinates: 2209073..2209363 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: I4ID81
• Locus tag: myaer102_RS11375
• Protein ID: WP_002757266.1
• Coordinates: 2209373..2209663 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
myaer102_RS11335	2204342..2204962	-	621	Protein_2116	agmatinase	-
myaer102_RS11345	2205359..2205853	-	495	WP_125730771.1	ATPase	-
myaer102_RS11350	2206088..2206438	-	351	WP_004162509.1	DUF86 domain-containing protein	-
myaer102_RS11355	2206428..2206718	-	291	WP_002757283.1	nucleotidyltransferase	-
myaer102_RS11360	2206855..2207352	-	498	WP_012267264.1	hypothetical protein	-
myaer102_RS30675	2208283..2208411	+	129	Protein_2121	PEP-CTERM sorting domain-containing protein	-
myaer102_RS30680	2208593..2208844	+	252	Protein_2122	DUF2442 domain-containing protein	-
myaer102_RS11370	2209073..2209363	+	291	WP_008201144.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
myaer102_RS11375	2209373..2209663	+	291	WP_002757266.1	putative addiction module antidote protein	Antitoxin
myaer102_RS11380	2209634..2209825	-	192	WP_125730772.1	hypothetical protein	-
myaer102_RS11385	2209953..2210753	+	801	WP_008201141.1	uroporphyrinogen-III C-methyltransferase	-
myaer102_RS11390	2210764..2211183	+	420	WP_008201139.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
myaer102_RS11395	2211316..2211810	+	495	WP_002748906.1	photosystem I reaction center subunit III	-
myaer102_RS11400	2211856..2211984	+	129	WP_002738148.1	photosystem I reaction center subunit IX	-
myaer102_RS11405	2212151..2213752	+	1602	WP_125732768.1	carboxylate-amine ligase	-
myaer102_RS11410	2213883..2214095	-	213	WP_002777112.1	DUF3148 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2196052..2209825	13773

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (7-92)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10990.56 Da
• Isoelectric Point: 9.8618

>T32628 WP_008201144.1 NZ_AP019314:2209073-2209363 [Microcystis viridis NIES-102]
MIEIRQTETYSQWFSNLRDHQAKARIDIRVRRLSMGNPGDVKPVGKGVSELRIDYGPGYRVYFIQRGETLIILLAGGDKQ
TQERDIKTALNLAQDL

Nucleotide

• Length: 291 bp

>T32628 NZ_AP019314:2209073-2209363 [Microcystis viridis NIES-102]
ATGATTGAAATTCGCCAGACCGAAACCTACTCTCAGTGGTTTAGCAATTTGCGGGATCACCAAGCAAAAGCACGTATAGA
CATTCGTGTTCGTCGCCTGTCTATGGGCAATCCAGGCGATGTTAAACCAGTAGGAAAAGGTGTATCGGAACTGCGGATCG
ATTATGGCCCAGGCTATCGGGTGTATTTCATTCAGCGAGGCGAGACCTTGATCATCCTCTTGGCAGGAGGAGATAAGCAA
ACTCAAGAACGGGACATCAAAACAGCATTAAATTTAGCACAAGACTTATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10320.80 Da
• Isoelectric Point: 4.4475

>AT32628 WP_002757266.1 NZ_AP019314:2209373-2209663 [Microcystis viridis NIES-102]
MATIQTYPWDAAEHLETKEDIAAYLEAALEDGDPSLVVAALGDIARSKGMTHIARETGLGRESLYKALSIEGNPEFATVL
KVLQSLGLRLQVVPIA

Nucleotide

• Length: 291 bp

>AT32628 NZ_AP019314:2209373-2209663 [Microcystis viridis NIES-102]
ATGGCTACTATCCAAACCTATCCTTGGGATGCGGCAGAACATCTCGAAACAAAAGAAGATATAGCTGCATATCTTGAAGC
AGCTCTTGAAGATGGCGATCCAAGCTTAGTGGTAGCAGCATTAGGTGATATTGCTCGATCTAAAGGGATGACACATATTG
CCCGTGAAACAGGGTTGGGGCGTGAAAGTCTCTATAAAGCTTTGTCAATCGAAGGCAATCCAGAGTTTGCCACAGTTCTT
AAGGTCTTACAATCACTTGGGTTACGCCTCCAAGTCGTACCAATTGCCTAA

Structures

Toxin

• AlphaFold DB: I4ID82

Antitoxin

• AlphaFold DB: I4ID81