Detailed information of TA system TA032626

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 697214..698073
• Replicon: chromosome
• Accession: NZ_AP019314
• Organism: Microcystis viridis NIES-102

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: I4HG17
• Locus tag: myaer102_RS03580
• Protein ID: WP_002791882.1
• Coordinates: 697675..698073 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: -
• Locus tag: myaer102_RS03575
• Protein ID: WP_125730342.1
• Coordinates: 697214..697633 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
myaer102_RS03560	692677..693846	+	1170	WP_012266038.1	trypsin-like peptidase domain-containing protein	-
myaer102_RS03565	693915..694778	-	864	WP_012266039.1	WYL domain-containing protein	-
myaer102_RS03570	694878..697043	+	2166	WP_125730341.1	type I-D CRISPR-associated helicase Cas3'	-
myaer102_RS03575	697214..697633	+	420	WP_125730342.1	nucleotidyltransferase domain-containing protein	Antitoxin
myaer102_RS03580	697675..698073	+	399	WP_002791882.1	DUF86 domain-containing protein	Toxin
myaer102_RS03590	698473..701916	+	3444	WP_125730344.1	type I-D CRISPR-associated protein Cas10d/Csc3	-
myaer102_RS03595	701934..702941	+	1008	WP_125730345.1	type I-D CRISPR-associated protein Cas7/Csc2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (1-117)

Antitoxin

• Polymerase beta, nucleotidyltransferase (6-101)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15326.56 Da
• Isoelectric Point: 4.6345

>T32626 WP_002791882.1 NZ_AP019314:697675-698073 [Microcystis viridis NIES-102]
MNQYLAELSEYGSITLEDYRTLRERQLAIERLIQLIVQTGIDINYQILKCLDIESPNNARDALFQIVELGILEEHLAVQL
AESIKLRNLLVHLYKKIDPDIVHSSIANILRDYPRYQRSIVQYLDSLEAENG

Nucleotide

• Length: 399 bp

>T32626 NZ_AP019314:697675-698073 [Microcystis viridis NIES-102]
ATGAATCAATACCTCGCCGAACTTAGCGAGTACGGTTCGATCACCTTAGAGGATTATCGTACATTAAGAGAACGTCAATT
AGCCATAGAAAGGTTAATTCAGCTTATCGTTCAAACTGGCATCGATATCAATTACCAGATTTTAAAATGTTTAGATATCG
AGTCTCCGAATAATGCTCGCGACGCATTATTTCAAATCGTAGAACTGGGAATTTTAGAAGAACACCTAGCCGTTCAACTG
GCCGAGTCTATTAAATTAAGAAACTTACTCGTACACCTATACAAGAAAATTGATCCCGATATCGTTCATTCCTCGATCGC
TAATATTTTACGAGATTATCCCCGCTACCAACGCTCGATCGTTCAATATCTAGATTCCTTGGAGGCAGAAAATGGTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 16109.51 Da
• Isoelectric Point: 4.7739

>AT32626 WP_125730342.1 NZ_AP019314:697214-697633 [Microcystis viridis NIES-102]
MNRLVEKITAIVEKLPNLKLLILFGSRARGDHRPDSDWDFAVLYEEGSDQKDISSLLKIYTLLEQALEIPEDKIDVIDLK
ECSPILAHYVARDGQLLYERETGLFEGFKEKFLMNPEESKALYRQLRNNLEISLREKGV

Nucleotide

• Length: 420 bp

>AT32626 NZ_AP019314:697214-697633 [Microcystis viridis NIES-102]
ATGAATAGGCTAGTAGAAAAAATTACGGCTATTGTCGAAAAACTACCGAATTTAAAACTATTAATTCTCTTCGGTTCTAG
AGCGCGAGGCGATCATCGACCCGATAGCGATTGGGATTTCGCGGTGCTATACGAGGAAGGAAGCGATCAAAAGGATATTT
CGAGTCTGTTGAAAATTTATACACTACTCGAACAAGCTTTAGAGATTCCCGAGGACAAAATTGATGTGATTGATTTAAAA
GAATGTTCGCCGATCCTCGCTCATTATGTCGCGCGGGATGGTCAACTTCTATACGAACGGGAAACGGGACTATTCGAGGG
ATTTAAAGAAAAATTTCTCATGAATCCTGAAGAATCGAAAGCGTTATACCGTCAATTACGGAATAATCTGGAGATTTCTC
TACGAGAAAAAGGAGTGTAA

Structures

Toxin

• AlphaFold DB: I4HG17