Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 161562..162010 Replicon chromosome
Accession NZ_AP019314
Organism Microcystis viridis NIES-102

Toxin (Protein)


Gene name hicA Uniprot ID B0JHA8
Locus tag myaer102_RS00775 Protein ID WP_012265576.1
Coordinates 161816..162010 (-) Length 65 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID I4GA01
Locus tag myaer102_RS00770 Protein ID WP_002771759.1
Coordinates 161562..161819 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
myaer102_RS00745 156973..157752 - 780 WP_002796685.1 photosystem II S4 domain protein -
myaer102_RS00750 157824..158333 - 510 WP_002796683.1 hypothetical protein -
myaer102_RS00755 158387..159721 - 1335 WP_125730179.1 transposase -
myaer102_RS00760 160041..160238 - 198 WP_125730180.1 hypothetical protein -
myaer102_RS00765 160461..161318 - 858 WP_002732125.1 IS5-like element ISMae4 family transposase -
myaer102_RS00770 161562..161819 - 258 WP_002771759.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
myaer102_RS00775 161816..162010 - 195 WP_012265576.1 type II toxin-antitoxin system HicA family toxin Toxin
myaer102_RS00780 162314..163534 - 1221 WP_125730181.1 efflux RND transporter periplasmic adaptor subunit -
myaer102_RS00785 163578..163799 - 222 WP_002749922.1 DUF4327 family protein -
myaer102_RS00790 163968..164855 - 888 WP_002796678.1 acetylglutamate kinase -
myaer102_RS00795 164935..166158 - 1224 WP_002796677.1 amidohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 160461..161354 893


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(6-62)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7280.49 Da        Isoelectric Point: 10.7019

>T32623 WP_012265576.1 NZ_AP019314:c162010-161816 [Microcystis viridis NIES-102]
MKVRDVLKRLEADGWYQIRMRGSHRILAHETKPGIVVVPGKSGDDIPKGTLASIWKQAQLEDKR

Download         Length: 195 bp

>T32623 NZ_AP019314:c162010-161816 [Microcystis viridis NIES-102]
TTGAAAGTTCGTGATGTACTCAAACGATTAGAAGCTGATGGTTGGTATCAGATCCGAATGCGCGGGAGCCATCGTATCCT
TGCCCATGAAACAAAGCCAGGAATCGTGGTTGTTCCTGGCAAGTCTGGGGACGACATACCGAAAGGAACATTAGCATCGA
TTTGGAAACAAGCTCAATTGGAGGATAAACGATGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9297.57 Da        Isoelectric Point: 3.9826

>AT32623 WP_002771759.1 NZ_AP019314:c161819-161562 [Microcystis viridis NIES-102]
MKEYAVIYEQGETNWGAMVPDLPGCVSIGDTFEEVQENIKEAIELYLEVLQERGETIPEPTTKVGLVGVAAGGFLPTTQK
LLGFH

Download         Length: 258 bp

>AT32623 NZ_AP019314:c161819-161562 [Microcystis viridis NIES-102]
ATGAAAGAGTATGCCGTCATTTACGAACAAGGCGAAACAAACTGGGGAGCAATGGTTCCCGACTTGCCCGGTTGCGTGAG
TATTGGAGATACCTTTGAAGAGGTTCAGGAAAATATTAAAGAAGCGATTGAACTTTACCTTGAAGTTTTGCAAGAACGCG
GGGAAACCATTCCAGAACCCACAACTAAAGTCGGATTGGTTGGAGTCGCTGCAGGAGGGTTCTTGCCGACTACCCAAAAA
CTGCTGGGGTTTCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9JIH3

Antitoxin

Source ID Structure
AlphaFold DB I4GA01

References