Detailed information of TA system TA032616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 6748348..6748982
• Replicon: chromosome
• Accession: NZ_AP019308
• Organism: Paenibacillus baekrokdamisoli strain KCTC 33723

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EKN16_RS29670
• Protein ID: WP_125664969.1
• Coordinates: 6748632..6748982 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: EKN16_RS29665
• Protein ID: WP_125664967.1
• Coordinates: 6748348..6748629 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKN16_RS29650	6743783..6745012	-	1230	WP_125664962.1	beta-glucanase	-
EKN16_RS32550	6745041..6745181	+	141	WP_164523050.1	hypothetical protein	-
EKN16_RS29655	6745444..6746649	+	1206	WP_125664963.1	DUF4367 domain-containing protein	-
EKN16_RS29660	6746920..6748116	+	1197	WP_125664965.1	alanine racemase	-
EKN16_RS29665	6748348..6748629	+	282	WP_125664967.1	ribbon-helix-helix protein, CopG family	Antitoxin
EKN16_RS29670	6748632..6748982	+	351	WP_125664969.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EKN16_RS29675	6749210..6749872	+	663	WP_125666789.1	ethanolamine utilization microcompartment protein EutL	-
EKN16_RS29680	6749920..6750255	+	336	WP_125666791.1	BMC domain-containing protein	-
EKN16_RS29685	6750307..6751830	+	1524	WP_125664971.1	acetaldehyde dehydrogenase (acetylating)	-
EKN16_RS29690	6751893..6752180	+	288	WP_125664973.1	BMC domain-containing protein	-
EKN16_RS29695	6752189..6752977	+	789	WP_125664975.1	phosphate propanoyltransferase	-
EKN16_RS29700	6752984..6753820	+	837	WP_125664977.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-46)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12806.72 Da
• Isoelectric Point: 5.1284

>T32616 WP_125664969.1 NZ_AP019308:6748632-6748982 [Paenibacillus baekrokdamisoli]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAASHGFDRDSVLLLEQI
RTIDKQRLTDKITHLDDETMRKVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T32616 NZ_AP019308:6748632-6748982 [Paenibacillus baekrokdamisoli]
TTGATCGTTAAACGCGGCGATGTATTTTTTGCTGACTTGTCACCTGTTGTCGGTTCAGAGCAGGGTGGCGTCCGTCCAGT
TCTTGTTATTCAGAATGATATCGGCAATCGCTTCAGTCCAACTGTGATTGTAGCTGCCATAACTGCACAAATTCAGAAGG
CCAAACTGCCGACGCATGTCGAAATTGACGCTGCGTCACACGGGTTTGACCGAGATTCTGTGCTGCTATTGGAGCAGATC
CGAACGATTGATAAACAGCGCTTGACCGATAAGATTACACACCTTGACGATGAGACGATGCGTAAGGTGGATGAGGCGCT
GCAAATCAGCGTTGGCTTAATTGATTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10607.24 Da
• Isoelectric Point: 7.0153

>AT32616 WP_125664967.1 NZ_AP019308:6748348-6748629 [Paenibacillus baekrokdamisoli]
VANLQNTKRIMISLPDHLLEEVDGIVAKENSNRSEFIRQAMKLYLIERKKRQIRESMQRGYLEMAKINLVMASEAFQAEE
DAGDTLGRLVSGV

Nucleotide

• Length: 282 bp

>AT32616 NZ_AP019308:6748348-6748629 [Paenibacillus baekrokdamisoli]
GTGGCCAATTTGCAAAACACCAAAAGGATAATGATCAGCCTGCCTGATCATTTGCTGGAGGAAGTCGACGGGATCGTTGC
CAAAGAAAACTCAAACCGAAGTGAATTTATTCGTCAGGCGATGAAGTTGTATTTAATCGAGCGTAAAAAGCGCCAAATTC
GCGAATCAATGCAGCGCGGTTATTTGGAAATGGCGAAAATCAACTTGGTGATGGCATCGGAAGCTTTTCAAGCGGAGGAA
GATGCCGGCGATACGCTCGGCCGACTCGTAAGCGGGGTGTAA