Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 843540..844054 Replicon chromosome
Accession NZ_AP019306
Organism Staphylococcus aureus strain TUM9463

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag EKM74_RS04725 Protein ID WP_000581792.1
Coordinates 843540..843749 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag EKM74_RS04730 Protein ID WP_000138932.1
Coordinates 843761..844054 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKM74_RS04705 839053..840411 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
EKM74_RS04710 840426..841496 - 1071 WP_042109557.1 D-alanine--D-alanine ligase -
EKM74_RS04715 841814..843011 + 1198 Protein_859 rod shape-determining protein RodA -
EKM74_RS04720 843275..843412 - 138 WP_000828354.1 hypothetical protein -
EKM74_RS04725 843540..843749 - 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
EKM74_RS04730 843761..844054 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
EKM74_RS04735 844220..845704 + 1485 WP_052999385.1 cardiolipin synthase -
EKM74_RS04740 845732..846379 + 648 WP_001187630.1 HD domain-containing protein -
EKM74_RS04765 847292..848164 - 873 WP_000725802.1 membrane protein insertase YidC -
EKM74_RS04770 848250..848891 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T32599 WP_000581792.1 NZ_AP019306:c843749-843540 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T32599 NZ_AP019306:c843749-843540 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT32599 WP_000138932.1 NZ_AP019306:c844054-843761 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT32599 NZ_AP019306:c844054-843761 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References