Detailed information of TA system TA032597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 768619..769097
• Replicon: chromosome
• Accession: NZ_AP019306
• Organism: Staphylococcus aureus strain TUM9463

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: A0A0H3K0C1
• Locus tag: EKM74_RS04335
• Protein ID: WP_001078344.1
• Coordinates: 768619..768945 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: -
• Locus tag: EKM74_RS04340
• Protein ID: WP_025175789.1
• Coordinates: 768948..769097 (-)
• Length: 50 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKM74_RS04310	763843..764184	-	342	WP_001190615.1	hypothetical protein	-
EKM74_RS04315	764634..765275	-	642	WP_063663566.1	pathogenicity island protein	-
EKM74_RS04320	765272..765652	-	381	WP_000356942.1	hypothetical protein	-
EKM74_RS04325	765962..767671	-	1710	WP_000447473.1	DUF927 domain-containing protein	-
EKM74_RS04330	767685..768554	-	870	WP_001002689.1	primase alpha helix C-terminal domain-containing protein	-
EKM74_RS04335	768619..768945	-	327	WP_001078344.1	DUF1474 family protein	Toxin
EKM74_RS04340	768948..769097	-	150	WP_025175789.1	hypothetical protein	Antitoxin
EKM74_RS04345	769150..769296	-	147	WP_000784885.1	hypothetical protein	-
EKM74_RS04350	769293..769610	-	318	WP_000481967.1	helix-turn-helix domain-containing protein	-
EKM74_RS04355	769615..769833	-	219	WP_000153640.1	helix-turn-helix transcriptional regulator	-
EKM74_RS04360	770006..770680	+	675	WP_000620857.1	helix-turn-helix transcriptional regulator	-
EKM74_RS04365	770694..771866	+	1173	WP_000179343.1	site-specific integrase	-
EKM74_RS04370	771935..773551	-	1617	WP_000240642.1	chaperonin GroEL	-
EKM74_RS04375	773627..773911	-	285	WP_000917289.1	co-chaperone GroES	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	aac(6')-aph(2'')	map / scn / chp / sak / see / hlb / tsst-1 / groEL / hld	659378..805230	145852

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12968.26 Da
• Isoelectric Point: 4.3050

>T32597 WP_001078344.1 NZ_AP019306:c768945-768619 [Staphylococcus aureus]
MNREIKDLFSDLKLLKDSFEDLKDSNGWHFDELYPYEPNHVLNKDELIGEGFSYHERRIHNNQMFDLFHLYIEQFDNIIE
KFYEIEKASSENFGEESDDAKNSIKVAE

Nucleotide

• Length: 327 bp

>T32597 NZ_AP019306:c768945-768619 [Staphylococcus aureus]
ATGAATCGGGAAATTAAAGATTTATTTAGCGATTTAAAGTTGTTGAAAGATAGTTTTGAGGATTTAAAGGATAGTAATGG
TTGGCATTTTGATGAGCTATATCCATATGAACCAAATCATGTCTTAAATAAAGATGAATTAATTGGAGAGGGATTTTCTT
ATCATGAGAGACGTATTCACAATAATCAAATGTTTGATTTATTCCATCTCTATATAGAGCAGTTCGATAATATTATCGAA
AAGTTTTATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGAATCAGATGACGCAAAGAATTCAATAAAAGTAGC
AGAGTAA

Antitoxin

Protein

• Length: 50 a.a.
• Molecular weight: 5822.52 Da
• Isoelectric Point: 4.4876

>AT32597 WP_025175789.1 NZ_AP019306:c769097-768948 [Staphylococcus aureus]
VNNLESYEHTEYVNGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 150 bp

>AT32597 NZ_AP019306:c769097-768948 [Staphylococcus aureus]
GTGAATAATTTGGAAAGTTATGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCA
CTTAGAATTGATAATGAAATGGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: A0A0H3K0C1