32575

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 849128..849642 Replicon chromosome
Accession NZ_AP019305
Organism Staphylococcus aureus strain TUM9458

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag EKM72_RS04755 Protein ID WP_000581792.1
Coordinates 849128..849337 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag EKM72_RS04760 Protein ID WP_000138932.1
Coordinates 849349..849642 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKM72_RS04735 844636..845994 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
EKM72_RS04740 846009..847079 - 1071 WP_042109557.1 D-alanine--D-alanine ligase -
EKM72_RS04745 847397..848599 + 1203 WP_001109939.1 rod shape-determining protein RodA -
EKM72_RS04750 848863..849000 - 138 WP_000828354.1 hypothetical protein -
EKM72_RS04755 849128..849337 - 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
EKM72_RS04760 849349..849642 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
EKM72_RS04765 849808..851292 + 1485 WP_052999385.1 cardiolipin synthase -
EKM72_RS04770 851320..851967 + 648 WP_001187630.1 HD domain-containing protein -
EKM72_RS04795 852880..853752 - 873 WP_000725802.1 membrane protein insertase YidC -
EKM72_RS04800 853838..854479 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T32575 WP_000581792.1 NZ_AP019305:c849337-849128 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T32575 NZ_AP019305:c849337-849128 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT32575 WP_000138932.1 NZ_AP019305:c849642-849349 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT32575 NZ_AP019305:c849642-849349 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References