Detailed information of TA system TA032574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 827869..828398
• Replicon: chromosome
• Accession: NZ_AP019305
• Organism: Staphylococcus aureus strain TUM9458

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EKM72_RS04655
• Protein ID: WP_000621175.1
• Coordinates: 827869..828231 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: EKM72_RS04660
• Protein ID: WP_000948331.1
• Coordinates: 828228..828398 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKM72_RS04630	824847..825617	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
EKM72_RS04635	825592..826071	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
EKM72_RS04640	826073..826399	-	327	WP_001052491.1	anti-sigma factor antagonist	-
EKM72_RS04645	826518..827519	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
EKM72_RS04655	827869..828231	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EKM72_RS04660	828228..828398	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
EKM72_RS04665	828483..829631	-	1149	WP_001281154.1	alanine racemase	-
EKM72_RS04670	829697..830056	-	360	WP_000581197.1	holo-ACP synthase	-
EKM72_RS04675	830060..830551	-	492	WP_001205912.1	PH domain-containing protein	-
EKM72_RS04680	830538..832121	-	1584	WP_001294626.1	PH domain-containing protein	-
EKM72_RS04685	832114..832593	-	480	WP_001287087.1	hypothetical protein	-
EKM72_RS04690	832801..833361	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T32574 WP_000621175.1 NZ_AP019305:c828231-827869 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T32574 NZ_AP019305:c828231-827869 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT32574 WP_000948331.1 NZ_AP019305:c828398-828228 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT32574 NZ_AP019305:c828398-828228 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1