Detailed information of TA system TA032560

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4600949..4602522
• Replicon: chromosome
• Accession: NZ_AP019195
• Organism: Aeromonas caviae strain GSH8M-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A3G9IWM9
• Locus tag: EJL47_RS21580
• Protein ID: WP_049637213.1
• Coordinates: 4600949..4602268 (-)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: EJL47_RS21585
• Protein ID: WP_017410478.1
• Coordinates: 4602268..4602522 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJL47_RS21565	4596853..4597617	+	765	WP_049637211.1	ATP-binding cassette domain-containing protein	-
EJL47_RS21570	4597649..4598551	+	903	WP_049637230.1	ABC transporter substrate-binding protein	-
EJL47_RS21575	4598548..4600530	+	1983	WP_049637212.1	Fe(3+)-hydroxamate ABC transporter permease FhuB	-
EJL47_RS21580	4600949..4602268	-	1320	WP_049637213.1	type II toxin-antitoxin system HipA family toxin	Toxin
EJL47_RS21585	4602268..4602522	-	255	WP_017410478.1	helix-turn-helix domain-containing protein	Antitoxin
EJL47_RS21590	4602755..4603096	-	342	WP_197725296.1	DUF86 domain-containing protein	-
EJL47_RS21595	4603169..4604319	+	1151	WP_102948979.1	IS3 family transposase	-
EJL47_RS21600	4604338..4604631	-	294	Protein_4135	site-specific integrase	-
EJL47_RS21605	4604794..4606155	-	1362	WP_010672278.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49559.53 Da
• Isoelectric Point: 6.5054

>T32560 WP_049637213.1 NZ_AP019195:c4602268-4600949 [Aeromonas caviae]
MANLAVYMNGYRVGTFTKTTSGAHQFQYHESWLAQPGSRPISLSMPLRHQAYRGDEAYNFFDNLLPDNTEVRNRVVARYQ
AASTQPFDLLSCIGQDSVGALQLVTEGHDVPDVRRIDYKALSDDELKQILTSYKSGIPLGMVREQEEFRISIAGVQEKTA
LLYLDGLWYLPLNATPTTHIIKLPIGKIESHSYSIDLSQSVENEYLCTLIAKEFGLPVPHCFMMQVGQVKALAVERFDRR
YAADRSWIMRLPQEDFCQVLNVPSARKYESHGGPGITDIMETLLGSATPEQDRYLFMKSQVLFWLLAATDGHAKNFSVFI
EPEGRFRLTPFYDILSVYPVFGGRGLNRRDAKLAMGLASSKGKKYSIEQIFPRHFFQTAKTVGFERSAMEEILTELANSV
DDVIERVTQQLPADFPDTISSTILEGLKARSARLIKGWD

Nucleotide

• Length: 1320 bp

>T32560 NZ_AP019195:c4602268-4600949 [Aeromonas caviae]
ATGGCCAATCTGGCTGTCTACATGAACGGCTATCGGGTAGGCACATTCACCAAAACTACCAGCGGTGCCCATCAGTTTCA
GTACCATGAGTCATGGCTAGCACAACCGGGCAGCCGTCCGATCTCACTCTCCATGCCATTGCGCCATCAGGCCTACCGGG
GCGATGAAGCCTATAACTTCTTCGACAACCTGCTGCCAGATAATACGGAGGTGAGGAACAGGGTTGTAGCACGCTATCAA
GCGGCATCAACTCAGCCCTTCGATCTGCTCAGTTGCATTGGCCAGGATAGCGTAGGGGCATTACAGCTGGTTACCGAAGG
ACACGATGTTCCAGATGTCAGGCGAATCGACTACAAGGCCCTTTCCGATGACGAGCTCAAGCAGATCCTGACCAGCTACA
AATCAGGAATCCCTTTGGGGATGGTCAGGGAACAAGAGGAGTTTCGGATCTCTATCGCCGGGGTGCAAGAAAAGACTGCG
CTGCTCTATCTCGATGGCCTTTGGTACCTGCCACTCAACGCAACGCCGACAACCCACATTATCAAACTCCCTATCGGCAA
GATTGAAAGCCACTCCTACTCGATAGACCTCTCCCAGAGCGTAGAAAACGAATATCTCTGCACTCTGATTGCCAAGGAGT
TTGGTCTACCCGTACCTCACTGCTTCATGATGCAGGTTGGCCAGGTCAAAGCACTGGCTGTTGAACGCTTCGACAGACGT
TACGCTGCCGATCGCAGTTGGATAATGCGCCTTCCCCAAGAAGATTTCTGTCAGGTATTGAATGTTCCGTCAGCACGGAA
ATACGAGAGCCATGGAGGACCAGGGATCACTGACATCATGGAAACCTTGCTGGGCTCTGCGACACCAGAACAGGATCGCT
ACCTGTTCATGAAATCTCAGGTCCTGTTCTGGCTGCTGGCAGCCACCGATGGCCATGCAAAGAACTTCTCGGTCTTTATT
GAACCGGAAGGGCGTTTTCGCCTGACCCCTTTTTACGACATCCTATCTGTCTATCCAGTGTTTGGCGGCCGAGGCCTCAA
CCGAAGGGACGCCAAGCTCGCTATGGGCCTAGCAAGCAGCAAGGGAAAGAAGTACTCCATTGAGCAGATATTCCCGCGTC
ATTTCTTCCAAACGGCAAAAACTGTGGGATTTGAGCGTTCTGCTATGGAAGAGATCCTGACAGAACTGGCCAACTCCGTT
GATGATGTTATTGAGCGCGTTACCCAGCAATTGCCAGCAGACTTCCCCGATACCATCAGCAGCACCATTCTAGAGGGCCT
AAAAGCCCGCTCAGCCAGGCTGATAAAGGGTTGGGATTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9293.62 Da
• Isoelectric Point: 7.9834

>AT32560 WP_017410478.1 NZ_AP019195:c4602522-4602268 [Aeromonas caviae]
MKVTSAKQLSACIKDARLSQKLSQGKVAEKVGIRQDTVSSFELNPESTKLETLFKILAALELSVDIKPRNESSESPASGW
KEEW

Nucleotide

• Length: 255 bp

>AT32560 NZ_AP019195:c4602522-4602268 [Aeromonas caviae]
ATGAAAGTAACCAGCGCCAAGCAGCTGAGCGCCTGTATCAAGGATGCCAGGCTTTCTCAAAAGCTCTCTCAAGGCAAAGT
GGCCGAGAAAGTGGGGATTAGGCAAGACACAGTCTCCAGCTTCGAGCTCAATCCTGAGTCCACCAAGCTGGAAACCCTGT
TCAAGATCCTCGCAGCACTCGAATTGTCAGTGGATATCAAACCCCGCAATGAGTCATCCGAGAGTCCGGCATCGGGCTGG
AAGGAGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A3G9IWM9