Detailed information of TA system TA032553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezA/zeta-couple_hipB
• Location: 1114089..1115335
• Replicon: chromosome
• Accession: NZ_AP019192
• Organism: Streptococcus pneumoniae strain ASP0581

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: EL334_RS05960
• Protein ID: WP_000405354.1
• Coordinates: 1114565..1115335 (+)
• Length: 257 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: B6ZCC4
• Locus tag: EL334_RS05955
• Protein ID: WP_000579612.1
• Coordinates: 1114089..1114565 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL334_RS05920	1109199..1109447	+	249	WP_001814254.1	hypothetical protein	-
EL334_RS05925	1109618..1110337	+	720	Protein_1096	type A chloramphenicol O-acetyltransferase	-
EL334_RS05935	1111133..1112056	-	924	WP_000417606.1	protein rep	-
EL334_RS05945	1112260..1113090	+	831	Protein_1098	alpha-glycosidase	-
EL334_RS05950	1113358..1113813	+	456	WP_025173400.1	hypothetical protein	-
EL334_RS05955	1114089..1114565	+	477	WP_000579612.1	helix-turn-helix domain-containing protein	Antitoxin
EL334_RS05960	1114565..1115335	+	771	WP_000405354.1	zeta toxin family protein	Toxin
EL334_RS05965	1115591..1116274	+	684	WP_000492698.1	LexA family transcriptional regulator	-
EL334_RS05970	1116277..1117692	+	1416	WP_000118332.1	Y-family DNA polymerase	-
EL334_RS05975	1117689..1118054	+	366	WP_000981955.1	hypothetical protein	-
EL334_RS05980	1118044..1118334	+	291	WP_001080184.1	hypothetical protein	-
EL334_RS05985	1118629..1118985	+	357	WP_000827835.1	hypothetical protein	-
EL334_RS05990	1118990..1119355	+	366	WP_000401236.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	erm(B) / tet(M) / cat(pC194)	-	1040135..1128587	88452

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 257 a.a.
• Molecular weight: 29234.14 Da
• Isoelectric Point: 7.5403

>T32553 WP_000405354.1 NZ_AP019192:1114565-1115335 [Streptococcus pneumoniae]
VEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTELSHLGYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPKLSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVNNTRQLEELAIFERIQIYQRDRSCVYDSGEDKTSAATVLHDLLFGEWNQVEKEML
KSGEERLKDLTNRNGC

Nucleotide

• Length: 771 bp

>T32553 NZ_AP019192:1114565-1115335 [Streptococcus pneumoniae]
GTGGAAATCCAAGATTATACTGACAGTGAATTCAAACATGCTCTAGCACGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTCGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGAGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGATAGTGTAGAATACACCAAAGATTTTGCAGGTAAAATGGTAGAGTCTTTAGTAACAGAATTGAGTCA
TTTGGGATACAATCTTTTGATAGAGGGAACTTTACGAACAGTTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTAGCCTTGATTGCGACAAAACCTAAGCTGTCCTATCTGAGCACCCTTATCCGATACGAAGAA
CTATACGCTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTAATAA
TACACGACAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAG
GAGAAGATAAAACTTCAGCAGCAACCGTTCTTCATGATTTACTATTTGGAGAATGGAATCAAGTGGAGAAAGAGATGCTT
AAATCTGGAGAAGAAAGATTGAAAGATTTAACTAATCGAAATGGTTGTTAG

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18245.68 Da
• Isoelectric Point: 4.4188

>AT32553 WP_000579612.1 NZ_AP019192:1114089-1114565 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT32553 NZ_AP019192:1114089-1114565 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCCGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACGAGTTCAGTCTCTACCGAATTAATAGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGACTATGAATTGACTTTAAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTTTTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGCGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAACGGATGGTAGCCTAG

Structures

Antitoxin

• AlphaFold DB: B6ZCC4