Detailed information of TA system TA032546

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3675341..3676035
• Replicon: chromosome
• Accession: NZ_AP019189
• Organism: Escherichia coli strain M217

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: B7R44_RS18430
• Protein ID: WP_001094398.1
• Coordinates: 3675341..3675709 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: B7R44_RS18435
• Protein ID: WP_077249034.1
• Coordinates: 3675730..3676035 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7R44_RS18410	3670514..3671470	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
B7R44_RS18415	3671467..3673665	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
B7R44_RS18420	3673675..3674631	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
B7R44_RS18425	3674682..3675020	+	339	Protein_3447	LysR family transcriptional regulator	-
B7R44_RS18430	3675341..3675709	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
B7R44_RS18435	3675730..3676035	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B7R44_RS18440	3676139..3676783	-	645	WP_000086755.1	hypothetical protein	-
B7R44_RS18445	3676802..3677023	-	222	WP_000692345.1	DUF987 domain-containing protein	-
B7R44_RS18450	3677092..3677568	-	477	WP_001424026.1	RadC family protein	-
B7R44_RS18455	3677584..3678057	-	474	WP_000855059.1	antirestriction protein	-
B7R44_RS18465	3678399..3679220	-	822	WP_001234565.1	DUF945 domain-containing protein	-
B7R44_RS18475	3679321..3679529	-	209	Protein_3455	DUF905 domain-containing protein	-
B7R44_RS18480	3679630..3680085	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T32546 WP_001094398.1 NZ_AP019189:c3675709-3675341 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T32546 NZ_AP019189:c3675709-3675341 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT32546 WP_077249034.1 NZ_AP019189:c3676035-3675730 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT32546 NZ_AP019189:c3676035-3675730 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4