Detailed information of TA system TA032534

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1872946..1873781
• Replicon: chromosome
• Accession: NZ_AP019189
• Organism: Escherichia coli strain M217

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X9TM58
• Locus tag: B7R44_RS09595
• Protein ID: WP_000854761.1
• Coordinates: 1872946..1873323 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: B7R44_RS09600
• Protein ID: WP_001295723.1
• Coordinates: 1873413..1873781 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7R44_RS09555	1868335..1868517	-	183	WP_001016348.1	hypothetical protein	-
B7R44_RS09565	1868971..1869360	-	390	WP_001445118.1	IS110 family transposase	-
B7R44_RS09570	1869854..1870258	+	405	WP_000839179.1	IS66 family insertion sequence hypothetical protein	-
B7R44_RS09575	1870255..1870602	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
B7R44_RS09580	1870651..1872186	+	1536	WP_001333339.1	IS66-like element ISEc22 family transposase	-
B7R44_RS24605	1872621..1872734	-	114	WP_001161660.1	DUF957 domain-containing protein	-
B7R44_RS09590	1872747..1872949	-	203	Protein_1736	hypothetical protein	-
B7R44_RS09595	1872946..1873323	-	378	WP_000854761.1	TA system toxin CbtA family protein	Toxin
B7R44_RS09600	1873413..1873781	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B7R44_RS09605	1873944..1874165	-	222	WP_000692345.1	DUF987 domain-containing protein	-
B7R44_RS09610	1874228..1874704	-	477	WP_001186774.1	RadC family protein	-
B7R44_RS09615	1874720..1875193	-	474	WP_000855059.1	antirestriction protein	-
B7R44_RS09625	1875535..1876353	-	819	WP_001234729.1	DUF945 domain-containing protein	-
B7R44_RS09635	1876508..1876666	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14205.25 Da
• Isoelectric Point: 7.3249

>T32534 WP_000854761.1 NZ_AP019189:c1873323-1872946 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T32534 NZ_AP019189:c1873323-1872946 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT32534 WP_001295723.1 NZ_AP019189:c1873781-1873413 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT32534 NZ_AP019189:c1873781-1873413 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TM58

Antitoxin

• AlphaFold DB: S1NM52