Detailed information of TA system TA032523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1804446..1804932
• Replicon: chromosome
• Accession: NZ_AP019011
• Organism: Fluviibacter phosphoraccumulans strain SHINM1

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: SHINM1_RS08990
• Protein ID: WP_162049066.1
• Coordinates: 1804446..1804721 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: SHINM1_RS08995
• Protein ID: WP_162049065.1
• Coordinates: 1804705..1804932 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SHINM1_RS08960 (SHINM1_017660)	1799527..1799817	-	291	WP_162049071.1	co-chaperone GroES	-
SHINM1_RS08965 (SHINM1_017670)	1800054..1801436	+	1383	WP_211148910.1	Fic family protein	-
SHINM1_RS08970 (SHINM1_017680)	1801490..1802638	-	1149	WP_211148911.1	Fic family protein	-
SHINM1_RS08975 (SHINM1_017690)	1802790..1803092	+	303	WP_211148912.1	helix-turn-helix transcriptional regulator	-
SHINM1_RS08980 (SHINM1_017700)	1803061..1803807	-	747	WP_211148913.1	autoinducer binding domain-containing protein	-
SHINM1_RS08985 (SHINM1_017710)	1803905..1804429	+	525	WP_211148914.1	hypothetical protein	-
SHINM1_RS08990 (SHINM1_017720)	1804446..1804721	-	276	WP_162049066.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SHINM1_RS08995 (SHINM1_017730)	1804705..1804932	-	228	WP_162049065.1	ribbon-helix-helix protein, CopG family	Antitoxin
SHINM1_RS09000 (SHINM1_017740)	1805073..1805351	-	279	WP_211148915.1	hypothetical protein	-
SHINM1_RS11610	1805529..1806278	+	750	Protein_1748	YdcF family protein	-
SHINM1_RS09015 (SHINM1_017780)	1806322..1807167	-	846	WP_211148917.1	Wzz/FepE/Etk N-terminal domain-containing protein	-
SHINM1_RS09020 (SHINM1_017790)	1807295..1808077	+	783	WP_211148918.1	YdcF family protein	-
SHINM1_RS09025 (SHINM1_017800)	1808046..1808627	-	582	WP_211148919.1	plasmid pRiA4b ORF-3 family protein	-
SHINM1_RS09030	1808621..1809070	-	450	WP_211148920.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-35)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10601.41 Da
• Isoelectric Point: 10.2349

>T32523 WP_162049066.1 NZ_AP019011:c1804721-1804446 [Fluviibacter phosphoraccumulans]
MPWIIKYTESAQRQLKKLDKAAALRVLDYMDERIGILADPRSAGKNLVGPRMGSYWRYRVGDLRVICDIQDQTVVILVIE
IGHRREVYRGR

Nucleotide

• Length: 276 bp

>T32523 NZ_AP019011:c1804721-1804446 [Fluviibacter phosphoraccumulans]
TTGCCATGGATCATTAAATATACCGAGTCTGCGCAGCGGCAGCTTAAGAAGCTGGACAAGGCGGCTGCGCTGCGGGTCCT
CGATTATATGGATGAGCGGATCGGTATTCTGGCTGACCCGAGAAGCGCAGGTAAAAATCTAGTTGGGCCACGGATGGGTT
CATATTGGCGGTATCGGGTTGGGGATCTGCGGGTGATCTGCGATATTCAGGATCAAACCGTTGTTATTCTGGTCATTGAG
ATCGGCCATCGGCGAGAGGTGTATCGGGGGCGGTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8464.55 Da
• Isoelectric Point: 4.5084

>AT32523 WP_162049065.1 NZ_AP019011:c1804932-1804705 [Fluviibacter phosphoraccumulans]
MAVSLRLDPEIEQRLDHLAAQTGRAKSYYLRELIEGGLDDLEGFYLADATMERVRKGEESVLDAAEVRKSIAMDH

Nucleotide

• Length: 228 bp

>AT32523 NZ_AP019011:c1804932-1804705 [Fluviibacter phosphoraccumulans]
ATGGCGGTGTCGCTCAGGCTGGACCCTGAAATTGAACAACGGCTCGATCATTTGGCGGCCCAGACCGGGCGTGCCAAGTC
TTACTATTTACGCGAGCTGATCGAGGGTGGCCTCGATGATCTAGAAGGTTTTTACCTGGCGGATGCCACGATGGAGCGTG
TTCGCAAAGGTGAGGAGTCTGTGCTGGATGCGGCTGAAGTAAGGAAGTCGATTGCCATGGATCATTAA