Detailed information of TA system TA032520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 814130..814731
• Replicon: chromosome
• Accession: NZ_AP019011
• Organism: Fluviibacter phosphoraccumulans strain SHINM1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: SHINM1_RS04125
• Protein ID: WP_162050014.1
• Coordinates: 814130..814435 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: SHINM1_RS04130
• Protein ID: WP_162050013.1
• Coordinates: 814432..814731 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SHINM1_RS04085 (SHINM1_008080)	809594..809914	+	321	WP_162050019.1	chaperone modulator CbpM	-
SHINM1_RS04095 (SHINM1_008090)	810107..810397	+	291	WP_162050018.1	nucleotidyltransferase family protein	-
SHINM1_RS04100 (SHINM1_008100)	810394..810738	+	345	WP_202930671.1	DUF86 domain-containing protein	-
SHINM1_RS04105 (SHINM1_008120)	810942..812089	+	1148	WP_162050017.1	IS3 family transposase	-
SHINM1_RS04110 (SHINM1_008130)	812118..812783	-	666	WP_162050016.1	hypothetical protein	-
SHINM1_RS04115 (SHINM1_008140)	813129..813404	+	276	WP_162050015.1	hypothetical protein	-
SHINM1_RS04125 (SHINM1_008160)	814130..814435	+	306	WP_162050014.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SHINM1_RS04130 (SHINM1_008170)	814432..814731	+	300	WP_162050013.1	putative addiction module antidote protein	Antitoxin
SHINM1_RS04135 (SHINM1_008180)	814755..815066	-	312	WP_162050012.1	hypothetical protein	-
SHINM1_RS04140 (SHINM1_008190)	815071..816288	-	1218	WP_162050011.1	MFS transporter	-
SHINM1_RS04145 (SHINM1_008200)	816443..817180	+	738	WP_162050010.1	3-ketoacyl-ACP reductase	-
SHINM1_RS04150 (SHINM1_008210)	817262..817684	-	423	WP_162050942.1	DUF1841 family protein	-
SHINM1_RS04155 (SHINM1_008220)	817701..818360	-	660	WP_162050009.1	endonuclease III	-
SHINM1_RS04160 (SHINM1_008230)	818373..819062	-	690	WP_174237203.1	electron transport complex subunit E	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-92)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11439.08 Da
• Isoelectric Point: 9.9574

>T32520 WP_162050014.1 NZ_AP019011:814130-814435 [Fluviibacter phosphoraccumulans]
MKLIYTTEIFDAWFGRLKDKEVARRIQIRIDRAEDGHFGDCKSVGGGVSEMRIHHGPGYRVYFMQRGFEVVILLAGGDKS
SQSRDIQTAIHIAQQIKGSRS

Nucleotide

• Length: 306 bp

>T32520 NZ_AP019011:814130-814435 [Fluviibacter phosphoraccumulans]
ATGAAACTTATCTATACGACAGAAATTTTTGATGCTTGGTTTGGCCGATTAAAAGACAAAGAAGTTGCGCGGCGGATTCA
GATTCGGATCGACAGAGCAGAAGATGGTCATTTCGGAGACTGTAAGTCTGTTGGTGGCGGTGTTTCAGAAATGCGAATTC
ATCATGGGCCGGGATATCGGGTGTATTTCATGCAACGAGGATTCGAGGTGGTGATTTTACTAGCAGGTGGTGACAAGTCA
TCACAGTCCAGAGATATTCAAACAGCTATACATATAGCTCAGCAGATTAAGGGTAGCAGATCATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10721.36 Da
• Isoelectric Point: 7.2776

>AT32520 WP_162050013.1 NZ_AP019011:814432-814731 [Fluviibacter phosphoraccumulans]
MKAVKLRRWDSAEHLKTSEEMALYLEACFQEAGDDAAFIAKALGNIARAKGMSQLAQETGMGRESLYKALSGEGNPSFAT
ILKVTRALGIELHAMPVHA

Nucleotide

• Length: 300 bp

>AT32520 NZ_AP019011:814432-814731 [Fluviibacter phosphoraccumulans]
ATGAAAGCAGTAAAGCTACGTAGATGGGATAGCGCCGAACATCTGAAAACATCAGAAGAGATGGCGTTGTATCTAGAAGC
TTGCTTCCAAGAAGCAGGTGATGATGCTGCATTTATTGCCAAAGCACTGGGTAACATTGCACGTGCCAAAGGCATGTCAC
AGCTAGCCCAAGAGACCGGTATGGGACGGGAGAGTCTTTATAAAGCGCTCTCCGGAGAAGGTAACCCAAGCTTTGCAACC
ATTCTCAAGGTGACACGTGCTTTAGGTATTGAGCTTCATGCGATGCCAGTTCATGCCTAG