Detailed information of TA system TA032519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 630007..631445
• Replicon: chromosome
• Accession: NZ_AP019011
• Organism: Fluviibacter phosphoraccumulans strain SHINM1

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: SHINM1_RS03210
• Protein ID: WP_162050167.1
• Coordinates: 630007..630846 (-)
• Length: 280 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: SHINM1_RS03215
• Protein ID: WP_162050166.1
• Coordinates: 630843..631445 (-)
• Length: 201 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SHINM1_RS03180 (SHINM1_006160)	625336..625896	-	561	WP_162050173.1	hypothetical protein	-
SHINM1_RS03185 (SHINM1_006170)	625907..627019	-	1113	WP_244660485.1	TolC family protein	-
SHINM1_RS03190	627310..627684	-	375	WP_162050171.1	hypothetical protein	-
SHINM1_RS03195 (SHINM1_006180)	627743..628972	-	1230	WP_162050170.1	IS256 family transposase	-
SHINM1_RS03200 (SHINM1_006190)	629326..629631	+	306	WP_162050169.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SHINM1_RS03205 (SHINM1_006200)	629624..629941	+	318	WP_162050168.1	putative addiction module antidote protein	-
SHINM1_RS03210 (SHINM1_006210)	630007..630846	-	840	WP_162050167.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
SHINM1_RS03215 (SHINM1_006220)	630843..631445	-	603	WP_162050166.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
SHINM1_RS03220 (SHINM1_006230)	631535..632557	-	1023	WP_162050165.1	hypothetical protein	-
SHINM1_RS03225 (SHINM1_006250)	633011..633154	+	144	WP_162071257.1	hypothetical protein	-
SHINM1_RS03230 (SHINM1_006260)	633251..634465	+	1215	WP_162071258.1	hypothetical protein	-
SHINM1_RS03235 (SHINM1_006270)	634571..635170	-	600	WP_162071259.1	class I SAM-dependent methyltransferase	-
SHINM1_RS03240 (SHINM1_006280)	635183..635569	-	387	WP_162071260.1	rhodanese-like domain-containing protein	-
SHINM1_RS03245 (SHINM1_006290)	635573..636103	-	531	WP_162071261.1	YeeE/YedE thiosulfate transporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	532284..723112	190828
	-	flank	IS/Tn	-	-	627743..628972	1229

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (34-262)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (17-53)

Sequences

Toxin

Protein

• Length: 280 a.a.
• Molecular weight: 31236.08 Da
• Isoelectric Point: 4.5766

>T32519 WP_162050167.1 NZ_AP019011:c630846-630007 [Fluviibacter phosphoraccumulans]
MINRAASVRARLLAKAKAESQDFSLVLTRYGLERLLYRLSVSSNKDNFLLKGALLFDLWFDVPLRPTRDIDLLGFGPAEE
PLVLGAFQEICQIECDDGIEFDLDTLKIEEIRKDANYSGLRISLTGYVDGARCPIQVDIGYGDAVTPAPETAEYPVMLPD
TPVPILRVYPRYTVIAEKLEALIFLGMINTRLKDYFDLWVILTTTELDQNLLGEAITATLVRRKTALPASMPVGLSEEFS
SDAQKIAQWNAFVKKNQLNAPSLDVVVTILRDRFAQFVS

Nucleotide

• Length: 840 bp

>T32519 NZ_AP019011:c630846-630007 [Fluviibacter phosphoraccumulans]
ATGATCAATCGTGCCGCATCTGTTCGCGCTCGTTTGTTGGCCAAAGCCAAGGCCGAGAGCCAGGATTTCTCATTGGTGCT
AACCCGCTACGGACTTGAGCGTCTGCTATACCGTTTGAGCGTGTCTTCGAACAAAGACAACTTTTTACTCAAGGGTGCTC
TGCTGTTCGATTTGTGGTTCGACGTGCCGCTACGCCCCACCCGCGATATCGATTTGCTTGGGTTTGGTCCAGCCGAAGAG
CCTTTGGTATTGGGAGCGTTTCAGGAGATATGCCAGATTGAGTGCGACGATGGCATTGAGTTTGATCTCGACACCCTGAA
AATCGAGGAAATTCGAAAGGACGCCAATTATTCCGGGCTACGTATCAGTTTGACTGGATACGTCGATGGGGCACGCTGCC
CCATCCAGGTCGACATCGGCTACGGTGACGCCGTAACGCCAGCGCCTGAAACAGCGGAATATCCAGTCATGTTGCCGGAC
ACCCCAGTGCCGATACTTCGGGTCTATCCTCGGTATACGGTCATCGCTGAGAAATTAGAAGCACTGATCTTTCTGGGAAT
GATCAATACCCGACTTAAGGACTATTTCGACTTATGGGTCATTCTCACTACTACTGAGTTAGACCAAAACTTACTCGGTG
AAGCTATTACCGCAACCTTGGTTCGCAGAAAAACGGCGCTTCCAGCATCGATGCCCGTGGGCCTGTCAGAGGAGTTTTCT
TCTGATGCCCAGAAGATCGCCCAGTGGAACGCATTCGTTAAGAAAAACCAGCTCAATGCGCCGAGCCTTGATGTCGTTGT
TACCATTTTGCGCGACAGGTTCGCTCAATTTGTAAGTTAA

Antitoxin

Protein

• Length: 201 a.a.
• Molecular weight: 22378.14 Da
• Isoelectric Point: 10.3044

>AT32519 WP_162050166.1 NZ_AP019011:c631445-630843 [Fluviibacter phosphoraccumulans]
MKRQAHPVHQLLTQKTLLRPKDAVSSGYSRIYLAQLAKRGALRKVGRGLYASPLRKESEESGLAEVAMKYPRAVVCLLSA
LSVHGLTTQAPHEVWVAVGSKDRAPKMDYPPLRVVRFGGETLTEGSEVKVVEGIPVQITNVPKTIADCFKFRNKIGLDVA
LEALKEAWRDKRVSIDDLWRYAQLCRVQNVMRPYLESLVA

Nucleotide

• Length: 603 bp

>AT32519 NZ_AP019011:c631445-630843 [Fluviibacter phosphoraccumulans]
ATGAAAAGGCAAGCACACCCCGTCCACCAGCTACTGACGCAAAAGACGCTACTCCGTCCCAAAGACGCGGTATCAAGCGG
CTATTCGCGTATTTATCTGGCTCAGCTGGCCAAACGTGGTGCACTACGTAAGGTCGGCCGAGGGCTGTATGCCTCCCCGC
TGCGCAAAGAGTCAGAAGAGTCCGGCCTCGCCGAAGTGGCCATGAAATATCCACGGGCCGTTGTCTGCCTGCTGTCTGCC
CTGTCTGTTCATGGGCTCACAACCCAGGCACCCCACGAAGTATGGGTGGCCGTGGGTAGTAAGGATCGCGCCCCAAAAAT
GGATTATCCACCTCTCCGAGTCGTTCGCTTCGGAGGAGAAACACTAACAGAGGGGTCCGAGGTGAAGGTGGTTGAGGGCA
TCCCTGTTCAAATCACCAATGTTCCCAAGACGATTGCAGATTGCTTCAAGTTCCGCAACAAGATTGGCCTGGACGTAGCA
CTGGAAGCGCTGAAGGAGGCTTGGCGGGATAAGCGCGTCAGCATCGATGATCTGTGGCGTTACGCACAGCTATGTCGCGT
ACAGAATGTCATGCGCCCCTACCTTGAAAGCCTGGTCGCATGA