Detailed information of TA system TA032518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 629326..629941
• Replicon: chromosome
• Accession: NZ_AP019011
• Organism: Fluviibacter phosphoraccumulans strain SHINM1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: SHINM1_RS03200
• Protein ID: WP_162050169.1
• Coordinates: 629326..629631 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: SHINM1_RS03205
• Protein ID: WP_162050168.1
• Coordinates: 629624..629941 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SHINM1_RS03180 (SHINM1_006160)	625336..625896	-	561	WP_162050173.1	hypothetical protein	-
SHINM1_RS03185 (SHINM1_006170)	625907..627019	-	1113	WP_244660485.1	TolC family protein	-
SHINM1_RS03190	627310..627684	-	375	WP_162050171.1	hypothetical protein	-
SHINM1_RS03195 (SHINM1_006180)	627743..628972	-	1230	WP_162050170.1	IS256 family transposase	-
SHINM1_RS03200 (SHINM1_006190)	629326..629631	+	306	WP_162050169.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SHINM1_RS03205 (SHINM1_006200)	629624..629941	+	318	WP_162050168.1	putative addiction module antidote protein	Antitoxin
SHINM1_RS03210 (SHINM1_006210)	630007..630846	-	840	WP_162050167.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
SHINM1_RS03215 (SHINM1_006220)	630843..631445	-	603	WP_162050166.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
SHINM1_RS03220 (SHINM1_006230)	631535..632557	-	1023	WP_162050165.1	hypothetical protein	-
SHINM1_RS03225 (SHINM1_006250)	633011..633154	+	144	WP_162071257.1	hypothetical protein	-
SHINM1_RS03230 (SHINM1_006260)	633251..634465	+	1215	WP_162071258.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	532284..723112	190828
	-	flank	IS/Tn	-	-	627743..628972	1229

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-85)

Antitoxin

• Cro/C1-type helix-turn-helix domain (51-94)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11471.35 Da
• Isoelectric Point: 9.9485

>T32518 WP_162050169.1 NZ_AP019011:629326-629631 [Fluviibacter phosphoraccumulans]
MYTVIQIPAFSDWLRAIKDRQTQVRLMKRLRKAQLGNLGDVKQVGSGVWEMREFFGPGWRMYYVQRGDLLIVMLGGGDKS
TQTADIAKAISLASLVEIEHD

Nucleotide

• Length: 306 bp

>T32518 NZ_AP019011:629326-629631 [Fluviibacter phosphoraccumulans]
ATGTACACCGTTATACAAATCCCGGCCTTTTCTGATTGGCTCCGAGCCATTAAAGATCGCCAGACACAGGTTCGTCTAAT
GAAGCGCCTTCGTAAAGCGCAATTAGGTAATCTGGGGGATGTTAAACAGGTGGGCAGTGGTGTTTGGGAGATGCGGGAGT
TTTTCGGCCCCGGCTGGCGCATGTACTACGTTCAGCGTGGGGATTTGTTGATCGTGATGCTGGGTGGTGGAGATAAATCT
ACTCAAACAGCAGACATTGCTAAAGCCATTTCTCTTGCGAGCCTGGTGGAGATTGAACATGACTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11516.30 Da
• Isoelectric Point: 5.1568

>AT32518 WP_162050168.1 NZ_AP019011:629624-629941 [Fluviibacter phosphoraccumulans]
MTKKIKLADCRPFDIAEYLDDETMITEYMNVVLEENDPAMLASALGDIARARGMSQLAKETGLSRESLYKGLSGDRIPST
ETLLKVIHALGFKLSVSKVDTVAHA

Nucleotide

• Length: 318 bp

>AT32518 NZ_AP019011:629624-629941 [Fluviibacter phosphoraccumulans]
ATGACTAAGAAGATTAAACTTGCCGATTGCCGACCATTTGATATTGCAGAATATCTGGACGACGAAACCATGATTACAGA
GTATATGAACGTTGTGCTTGAAGAGAACGACCCAGCGATGTTGGCTTCAGCCTTGGGCGATATCGCTCGTGCTCGTGGCA
TGTCACAACTTGCGAAAGAGACTGGCCTCAGCAGAGAAAGCTTATACAAGGGGTTGTCTGGTGACCGTATCCCAAGTACC
GAGACCCTGCTAAAAGTCATTCATGCGCTGGGATTCAAGCTATCAGTATCCAAGGTGGATACAGTCGCGCACGCTTAA