Detailed information of TA system TA032517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/AbrB(antitoxin)
• Location: 464629..465184
• Replicon: chromosome
• Accession: NZ_AP019011
• Organism: Fluviibacter phosphoraccumulans strain SHINM1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SHINM1_RS02345
• Protein ID: WP_162050318.1
• Coordinates: 464629..464964 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: SHINM1_RS02350
• Protein ID: WP_162050317.1
• Coordinates: 464954..465184 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SHINM1_RS02310 (SHINM1_004450)	459872..460594	-	723	WP_162050324.1	UDP-2,3-diacylglucosamine diphosphatase	-
SHINM1_RS02315 (SHINM1_004460)	460591..461088	-	498	WP_162050323.1	peptidylprolyl isomerase	-
SHINM1_RS02320 (SHINM1_004470)	461221..462612	+	1392	WP_162050322.1	cysteine--tRNA ligase	-
SHINM1_RS02325 (SHINM1_004480)	462605..463813	+	1209	WP_242451452.1	MFS transporter	-
SHINM1_RS02330 (SHINM1_004500)	463979..464212	+	234	WP_162050321.1	CopG family transcriptional regulator	-
SHINM1_RS02335 (SHINM1_004510)	464199..464456	+	258	WP_162050320.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SHINM1_RS02340	464462..464617	+	156	WP_162050319.1	hypothetical protein	-
SHINM1_RS02345	464629..464964	-	336	WP_162050318.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SHINM1_RS02350 (SHINM1_004520)	464954..465184	-	231	WP_162050317.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SHINM1_RS02355	465268..465504	+	237	WP_162050316.1	hypothetical protein	-
SHINM1_RS02360 (SHINM1_004530)	465630..466109	+	480	WP_162050315.1	hypothetical protein	-
SHINM1_RS02365 (SHINM1_004540)	466115..466444	+	330	WP_162050314.1	hypothetical protein	-
SHINM1_RS02370 (SHINM1_004550)	466775..467326	+	552	WP_162050313.1	hypothetical protein	-
SHINM1_RS02375 (SHINM1_004560)	467440..468585	-	1146	WP_162050312.1	molecular chaperone DnaJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-102)

Antitoxin

• SpoVT-AbrB domain (6-47)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12292.22 Da
• Isoelectric Point: 10.7556

>T32517 WP_162050318.1 NZ_AP019011:c464964-464629 [Fluviibacter phosphoraccumulans]
VGFEQFDVVRVPFPFTDRTATKNRPALVLSQSVFSNAADHSVLVMVTSAMNDWPLDCPIKDLQAAGLPAPSRIRFKLFTL
DNRLIRGKIGALSAADRQAFKKQFDRLVATS

Nucleotide

• Length: 336 bp

>T32517 NZ_AP019011:c464964-464629 [Fluviibacter phosphoraccumulans]
GTGGGCTTTGAACAATTCGACGTGGTCAGAGTGCCCTTCCCGTTCACGGATCGTACTGCTACTAAAAACAGACCTGCACT
GGTTCTCTCTCAATCCGTCTTTAGTAATGCAGCAGACCATTCAGTGCTTGTGATGGTCACCTCGGCGATGAATGATTGGC
CTTTGGACTGCCCCATAAAAGACCTGCAAGCCGCAGGTTTGCCAGCACCCTCAAGAATCCGCTTCAAACTCTTCACGCTC
GACAACAGACTGATACGAGGAAAGATTGGTGCACTTTCCGCCGCTGATAGACAAGCATTCAAAAAGCAATTTGATCGATT
GGTCGCAACAAGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8480.50 Da
• Isoelectric Point: 4.1954

>AT32517 WP_162050317.1 NZ_AP019011:c465184-464954 [Fluviibacter phosphoraccumulans]
MPAIAKITSKGQTTIPAEVRESLNFNPGDLLEWEVNADGSARVRRIQPLDLEYMRAVEGTLTEWGCAEDEEAYRGL

Nucleotide

• Length: 231 bp

>AT32517 NZ_AP019011:c465184-464954 [Fluviibacter phosphoraccumulans]
ATGCCAGCTATCGCCAAAATCACCTCAAAAGGCCAAACTACCATTCCAGCAGAGGTCAGAGAGTCGCTTAACTTTAATCC
GGGCGATTTGCTGGAATGGGAAGTGAATGCAGACGGTTCAGCCAGAGTGCGGCGTATTCAACCACTAGACCTAGAGTACA
TGCGCGCCGTTGAAGGCACGCTCACCGAATGGGGCTGCGCGGAAGACGAAGAGGCGTATCGTGGGCTTTGA