Detailed information of TA system TA032515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 200657..201138
• Replicon: chromosome
• Accession: NZ_AP019011
• Organism: Fluviibacter phosphoraccumulans strain SHINM1

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: -
• Locus tag: SHINM1_RS01010
• Protein ID: WP_211149080.1
• Coordinates: 200857..201138 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: -
• Locus tag: SHINM1_RS01005
• Protein ID: WP_211149079.1
• Coordinates: 200657..200857 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SHINM1_RS00970 (SHINM1_001870)	196200..196961	-	762	WP_211149072.1	hypothetical protein	-
SHINM1_RS00975 (SHINM1_001880)	197180..197440	+	261	WP_211149073.1	helix-turn-helix transcriptional regulator	-
SHINM1_RS00980 (SHINM1_001890)	197447..197929	-	483	WP_211149074.1	hypothetical protein	-
SHINM1_RS00985 (SHINM1_001900)	197965..198573	-	609	WP_211149075.1	hypothetical protein	-
SHINM1_RS00990 (SHINM1_001910)	198588..198791	-	204	WP_211149076.1	hypothetical protein	-
SHINM1_RS00995 (SHINM1_001920)	199043..199318	-	276	WP_211149077.1	DUF6471 domain-containing protein	-
SHINM1_RS01000 (SHINM1_001930)	199442..200509	+	1068	WP_211149078.1	IS1595 family transposase	-
SHINM1_RS01005 (SHINM1_001940)	200657..200857	+	201	WP_211149079.1	DUF5397 domain-containing protein	Antitoxin
SHINM1_RS01010 (SHINM1_001950)	200857..201138	+	282	WP_211149080.1	virulence factor	Toxin
SHINM1_RS01015 (SHINM1_001960)	201201..201743	-	543	WP_211149081.1	hypothetical protein	-
SHINM1_RS01020 (SHINM1_001970)	201860..202027	-	168	WP_162050540.1	50S ribosomal protein L33	-
SHINM1_RS01025 (SHINM1_001980)	202056..202289	-	234	WP_162050539.1	50S ribosomal protein L28	-
SHINM1_RS01030 (SHINM1_001990)	202386..203066	-	681	WP_162050538.1	DNA repair protein RadC	-
SHINM1_RS01035 (SHINM1_002000)	203204..204430	+	1227	WP_242451462.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
SHINM1_RS01040 (SHINM1_002010)	204443..204892	+	450	WP_211149082.1	dUTP diphosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-86)

Antitoxin

• Protein of unknown function DUF5397 (5-66)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10615.05 Da
• Isoelectric Point: 5.6725

>T32515 WP_211149080.1 NZ_AP019011:200857-201138 [Fluviibacter phosphoraccumulans]
MYAVAFDLVVADAQQHHPKGVAQAYTEIGAILAERGFERVQGSLYVTENEDMANLFVAIQELRSRPWFPKSVRDIRAFRI
EQWSDFTSVVKTP

Nucleotide

• Length: 282 bp

>T32515 NZ_AP019011:200857-201138 [Fluviibacter phosphoraccumulans]
ATGTACGCCGTAGCCTTTGACTTAGTCGTCGCAGATGCCCAGCAACACCATCCAAAGGGAGTAGCACAGGCGTATACCGA
GATCGGTGCCATTCTTGCGGAACGCGGTTTCGAGCGGGTACAAGGCAGCTTGTATGTAACCGAAAACGAAGATATGGCTA
ATCTGTTCGTGGCGATCCAAGAGTTGCGTTCCCGACCCTGGTTTCCGAAGTCGGTGCGTGATATTCGCGCCTTCCGTATT
GAGCAGTGGTCAGACTTTACGTCAGTGGTAAAAACGCCGTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7417.45 Da
• Isoelectric Point: 4.2850

>AT32515 WP_211149079.1 NZ_AP019011:200657-200857 [Fluviibacter phosphoraccumulans]
MQVAIVPPRVPVGQIKSFGPIGPKYEVGNPLRQLDDGDWMVEVTMVETGEKAEYRLTYLNDDPEAR

Nucleotide

• Length: 201 bp

>AT32515 NZ_AP019011:200657-200857 [Fluviibacter phosphoraccumulans]
ATGCAAGTAGCAATCGTACCGCCACGAGTTCCTGTGGGTCAAATCAAGTCCTTTGGTCCTATTGGACCGAAGTACGAGGT
TGGCAATCCGCTGCGCCAGTTGGATGACGGCGACTGGATGGTTGAGGTGACGATGGTCGAAACTGGCGAGAAAGCTGAAT
ATCGATTGACGTACCTTAACGATGACCCGGAGGCTCGCTAA