Detailed information of TA system TA032492

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3617016..3617636
• Replicon: chromosome
• Accession: NZ_AP019007
• Organism: Enterobacter oligotrophicus strain CCA6

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: EoCCA6_RS17325
• Protein ID: WP_152083694.1
• Coordinates: 3617016..3617234 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: W0BRV6
• Locus tag: EoCCA6_RS17330
• Protein ID: WP_020885187.1
• Coordinates: 3617262..3617636 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EoCCA6_RS17295	3613025..3613285	+	261	WP_152084475.1	type B 50S ribosomal protein L31	-
EoCCA6_RS17300	3613288..3613428	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
EoCCA6_RS17305	3613425..3614135	-	711	WP_152083690.1	GNAT family N-acetyltransferase	-
EoCCA6_RS17310	3614239..3615699	+	1461	WP_152083691.1	PLP-dependent aminotransferase family protein	-
EoCCA6_RS17315	3615671..3616138	-	468	WP_152083692.1	hypothetical protein	-
EoCCA6_RS17320	3616255..3616806	-	552	WP_152083693.1	maltose O-acetyltransferase	-
EoCCA6_RS17325	3617016..3617234	-	219	WP_152083694.1	hemolysin expression modulator Hha	Toxin
EoCCA6_RS17330	3617262..3617636	-	375	WP_020885187.1	Hha toxicity modulator TomB	Antitoxin
EoCCA6_RS17335	3618139..3621285	-	3147	WP_152083695.1	multidrug efflux RND transporter permease subunit AcrB	-
EoCCA6_RS17340	3621308..3622501	-	1194	WP_152083696.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8626.03 Da
• Isoelectric Point: 8.9008

>T32492 WP_152083694.1 NZ_AP019007:c3617234-3617016 [Enterobacter oligotrophicus]
MTEKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Nucleotide

• Length: 219 bp

>T32492 NZ_AP019007:c3617234-3617016 [Enterobacter oligotrophicus]
ATGACTGAAAAACCATTAACCAAGATTGATTATTTGATGCGCTTGCGACGTTGTCAGTCAATAGACACGCTTGAGCGTGT
AATTGAAAAAAATAAATACGAGCTTTCTGATAATGAATTGGCTGTATTTTATTCAGCTGCTGACCATCGTCTTGCTGAGC
TAACAATGAATAAACTGTATGATAAGATTCCTTCATCTGTCTGGAAGTTTGTTCGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14495.31 Da
• Isoelectric Point: 4.8886

>AT32492 WP_020885187.1 NZ_AP019007:c3617636-3617262 [Enterobacter oligotrophicus]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFINVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT32492 NZ_AP019007:c3617636-3617262 [Enterobacter oligotrophicus]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGCAACCATGGCTGGGTGAATGACCCAACGTCTGCAATCAATTTACAGCTAAATGAGTTGATAGAGC
ATATCGCGACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTGATTGAGCAAATTGACGAATACCTG
GATGACACCTTTATGTTGTTCAGTAGCTACGGCATTAACGCACAGGATTTGCAAAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCATCAACGTGAGCAGAGCTAACCCTGTTAGCCTTTCCTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A5C1BX74