Detailed information of TA system TA032490

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 2759179..2759774
• Replicon: chromosome
• Accession: NZ_AP019007
• Organism: Enterobacter oligotrophicus strain CCA6

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: EoCCA6_RS13195
• Protein ID: WP_152083021.1
• Coordinates: 2759472..2759774 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: EoCCA6_RS13190
• Protein ID: WP_152083020.1
• Coordinates: 2759179..2759466 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EoCCA6_RS13185	2757551..2759182	+	1632	WP_152083019.1	Na/Pi cotransporter family protein	-
EoCCA6_RS13190	2759179..2759466	-	288	WP_152083020.1	putative addiction module antidote protein	Antitoxin
EoCCA6_RS13195	2759472..2759774	-	303	WP_152083021.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EoCCA6_RS13200	2759971..2760840	+	870	WP_152083022.1	23S rRNA pseudouridine(2604) synthase RluF	-
EoCCA6_RS13205	2760841..2761113	-	273	WP_119937941.1	DUF3811 domain-containing protein	-
EoCCA6_RS13210	2761164..2762108	-	945	WP_152083023.1	ketopantoate/pantoate/pantothenate transporter PanS	-
EoCCA6_RS13215	2762199..2763548	-	1350	WP_152083024.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Uncharacterized protein HI1420 (3-89)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11320.18 Da
• Isoelectric Point: 9.6823

>T32490 WP_152083021.1 NZ_AP019007:c2759774-2759472 [Enterobacter oligotrophicus]
MKEIVQTESFRCWEQNLTDRRAKTIIASRLFRLANGLTGDVKPVGEGISELRVHFGPGYRIYFKQQGDCIIVLLSGGDKS
SQNKDILMAKMLAKTFILQE

Nucleotide

• Length: 303 bp

>T32490 NZ_AP019007:c2759774-2759472 [Enterobacter oligotrophicus]
ATGAAGGAGATCGTTCAGACAGAGTCCTTCCGATGCTGGGAGCAAAACCTGACAGATCGGCGAGCAAAGACGATTATCGC
TTCACGGCTCTTTCGGCTGGCAAATGGCCTAACGGGCGATGTTAAACCCGTGGGTGAAGGAATAAGTGAACTAAGGGTTC
ACTTTGGTCCGGGCTACAGAATCTATTTTAAACAACAAGGTGATTGTATCATCGTGCTGTTAAGTGGCGGTGACAAAAGC
AGCCAGAATAAAGACATACTAATGGCAAAAATGCTGGCCAAAACATTCATACTGCAGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10104.50 Da
• Isoelectric Point: 5.0336

>AT32490 WP_152083020.1 NZ_AP019007:c2759466-2759179 [Enterobacter oligotrophicus]
MNKLTPYDPAEALVDDEEIAVFIADALETGDSAYIAKALGVVARAKGMSNISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGFGLSIKHAGD

Nucleotide

• Length: 288 bp

>AT32490 NZ_AP019007:c2759466-2759179 [Enterobacter oligotrophicus]
ATGAATAAATTAACCCCCTACGATCCCGCCGAAGCACTGGTTGATGACGAGGAAATCGCTGTATTTATAGCTGATGCGCT
AGAAACCGGTGACTCAGCGTATATTGCTAAAGCCCTGGGCGTTGTCGCGCGAGCCAAAGGAATGTCGAACATTTCACAGC
AAACCGGCTTATCACGAGAGCAACTTTATCGGTCATTCAGTGACAAGGGAAACCCAACGCTTAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGTTTTGGGCTATCAATCAAACACGCTGGAGATTAA